Summary for 4V5X
| Entry DOI | 10.2210/pdb4v5x/pdb |
| EMDB information | 2210 |
| Descriptor | SCAFFOLD STRAND,SCAFFOLD STRAND, STAPLE STRAND, ... (162 entities in total) |
| Functional Keywords | dna, dna-templated design, synthetic biology |
| Biological source | ENTEROBACTERIA PHAGE M13 (M13 PHAGE) More |
| Total number of polymer chains | 162 |
| Total formula weight | 4692024.27 |
| Authors | Bai, X.C.,Martin, T.G.,Scheres, S.H.W.,Dietz, H. (deposition date: 2012-10-09, release date: 2014-07-09, Last modification date: 2024-05-08) |
| Primary citation | Bai, X.,Martin, T.G.,Scheres, S.H.W.,Dietz, H. Cryo-Em Structure of a 3D DNA-Origami Object. Proc.Natl.Acad.Sci.USA, 109:20012-, 2012 Cited by PubMed Abstract: A key goal for nanotechnology is to design synthetic objects that may ultimately achieve functionalities known today only from natural macromolecular complexes. Molecular self-assembly with DNA has shown potential for creating user-defined 3D scaffolds, but the level of attainable positional accuracy has been unclear. Here we report the cryo-EM structure and a full pseudoatomic model of a discrete DNA object that is almost twice the size of a prokaryotic ribosome. The structure provides a variety of stable, previously undescribed DNA topologies for future use in nanotechnology and experimental evidence that discrete 3D DNA scaffolds allow the positioning of user-defined structural motifs with an accuracy that is similar to that observed in natural macromolecules. Thereby, our results indicate an attractive route to fabricate nanoscale devices that achieve complex functionalities by DNA-templated design steered by structural feedback. PubMed: 23169645DOI: 10.1073/PNAS.1215713109 PDB entries with the same primary citation |
| Experimental method | ELECTRON MICROSCOPY (11.5 Å) |
Structure validation
Download full validation report
