4V4J
Interactions and Dynamics of the Shine-Dalgarno Helix in the 70S Ribosome.
This is a non-PDB format compatible entry.
Summary for 4V4J
| Entry DOI | 10.2210/pdb4v4j/pdb |
| Related | 2QNH |
| Descriptor | 23S LARGE SUBUNIT RIBOSOMAL RNA, 50S ribosomal protein L13, 50S ribosomal protein L14, ... (54 entities in total) |
| Functional Keywords | 70s ribosome, shine-dalgarno, ribosome |
| Biological source | Thermus thermophilus More |
| Total number of polymer chains | 54 |
| Total formula weight | 2224546.50 |
| Authors | Korostelev, A.,Trakhanov, S.,Asahara, H.,Laurberg, M.,Noller, H.F. (deposition date: 2007-07-18, release date: 2014-07-09, Last modification date: 2024-10-09) |
| Primary citation | Korostelev, A.,Trakhanov, S.,Asahara, H.,Laurberg, M.,Lancaster, L.,Noller, H.F. Interactions and dynamics of the Shine Dalgarno helix in the 70S ribosome. Proc.Natl.Acad.Sci.Usa, 104:16840-16843, 2007 Cited by PubMed Abstract: The crystal structure of an initiation-like 70S ribosome complex containing an 8-bp Shine-Dalgarno (SD) helix was determined at 3.8-A resolution. Translation-libration-screw analysis showed that the inherent anisotropic motions of the SD helix were biased along its helical axis, suggesting that during the first step of translocation, the SD helix moves along its helical screw axis. Contacts between the SD helix and the ribosome were primarily through interactions with helices 23a, 26, and 28 of 16S rRNA. Contact with the neck (helix 28) of the 30S subunit near its hinge point suggests that formation of the SD helix could affect positioning of the head of the 30S subunit for optimal interaction with initiator tRNA. The bulged U723 in helix 23a interacts with the minor groove of the SD helix at the C1539.G-10 base pair, explaining its selective conservation in bacteria and archaea. PubMed: 17940016DOI: 10.1073/pnas.0707850104 PDB entries with the same primary citation |
| Experimental method | X-RAY DIFFRACTION (3.83 Å) |
Structure validation
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