4V4J

Interactions and Dynamics of the Shine-Dalgarno Helix in the 70S Ribosome.

This is a non-PDB format compatible entry.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 12.3.1
Synchrotron site	ALS
Beamline	12.3.1
Temperature [K]	100
Detector technology	CCD
Collection date	2004-07-01
Detector	ADSC QUANTUM 315
Wavelength(s)	0.9835
Spacegroup name	I 4 2 2
Unit cell lengths	507.210, 507.210, 692.510
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	30.000 - 3.830
R-factor	0.327
Rwork	0.327
R-free	0.35100
Starting model (for MR)	1VSA 2OW8. PROTEINS L15 L19 L21 L28 AND L29 FROM PDB ENTRY 2J03.
RMSD bond length	0.009
RMSD bond angle	1.237
Data reduction software	d*TREK
Data scaling software	SCALA
Phasing software	CNS
Refinement software	REFMAC (5.2.0019)

Data quality characteristics

	Overall
Low resolution limit [Å]	78.000
High resolution limit [Å]	3.830
Rmerge	0.150
Number of reflections	354761
Completeness [%]	82.0

Crystallization Conditions

crystal ID	method	pH	temperature	details
1		7.5	298	Buffer conditions: 25 mM K-HEPES (pH 7.5), 12.5 mM Na-cacodylate pH 5.5, 2.5 mM Tris-HCl (pH 7.5), 80 mM NH_4 Cl, 80 mM KCl, 11 mM MgCl_2, 6.5 mM Thermine HCl and 0.2 M KSCN. Crystallization by the hanging drop method over 26-30% MPD in the above buffer mix, using 2 uL of ribosome complex mixed with 2 uL of well mixture on siliconized cover slips, temperature 298K

Crystallization Reagents

ID	crystal ID	solution ID	reagent name	concentration	details
1	1	1	K-HEPES
10	1	1	H2O
11	1	2	K-HEPES
12	1	2	Na-cacodylate
13	1	2	Tris-HCl
14	1	2	NH_4 Cl
15	1	2	KCl
16	1	2	MgCl_2
17	1	2	Thermine HCl
18	1	2	KSCN
19	1	2	H2O
20	1	2	MPD
21	1	2	H2O
3	1	1	Na-cacodylate
4	1	1	Tris-HCl
5	1	1	NH_4 Cl
6	1	1	KCl
7	1	1	MgCl_2
8	1	1	Thermine HCl
9	1	1	KSCN