4V1W

3D structure of horse spleen apoferritin determined by electron cryomicroscopy

> Summary

Summary for 4V1W

EMDB informationEMD-2788
DescriptorFERRITIN LIGHT CHAIN
Functional Keywordsstorage protein, iron storage, iron transport, ferritins, apoferritins, horses, metals, spleen
Biological sourceEQUUS CABALLUS (HORSE)
Total number of polymer chains24
Total molecular weight476942.21
Authors
Russo, C.J.,Passmore, L.A. (deposition date: 2014-10-02, release date: 2014-12-10, modification date: 2014-12-31)
Primary citation
Russo, C.J.,Passmore, L.A.
Electron Microscopy. Ultrastable Gold Substrates for Electron Cryomicroscopy.
Science, 346:1377-, 2014
PubMed: 25504723
DOI: 10.1126/SCIENCE.1259530
MImport into Mendeley
Experimental method
ELECTRON MICROSCOPY (4.7 Å)
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Structure validation

ClashscoreRamachandran outliersSidechain outliers80.6%5.9%MetricValuePercentile RanksWorseBetterPercentile relative to all structuresPercentile relative to all EM structures

More Asymmetric unit images

Molmil generated image of 4v1w
no rotation
Molmil generated image of 4v1w
rotated about x axis by 90°
Molmil generated image of 4v1w
rotated about y axis by 90°

> Structural details

Entity

Chain IDDescriptionTypeChain lengthFormula weightNumber of moleculesDB Name (Accession)Biological sourceDescriptive keywords
A, B, C, D, E...FERRITIN LIGHT CHAINpolymer17419872.624
UniProt (P02791)
Pfam (PF00210)
EQUUS CABALLUS (HORSE)@PDBjFERRITIN L SUBUNIT

Sequence viewer

Contents of the asymmetric unit

PolymersNumber of chains24
Total molecular weight476942.2
Non-Polymers*Number of molecules0
Total molecular weight0.0
All*Total molecular weight476942.2
*Water molecules are not included.

> Experimental details

Refinement Statistics

Experimental method:ELECTRON MICROSCOPY (4.7 Å)

Cell axes1.0001.0001.000
Cell angles90.0090.0090.00
SpacegroupP 1
Resolution limits - 4.70
the highest resolution shell value -

Data Collection Statistics

Resolution limits -
the highest resolution shell value -

Crystallization Conditions

crystal IDmethodpHpH rangetemperatureunit

Crystallization Reagents

IDcrystal IDsolution IDreagent nameconcentrationdetails
Crystallization Reagents in Literatures*
IDcrystal IDsolutionreagent nameconcentration (unit)details
Annotated Information is extracted from Literature Info*

> Functional details

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Functional Information from GO Data

ChainGOidnamespacecontents
A0005737cellular_componentcytoplasm
A0008043cellular_componentintracellular ferritin complex
A0008199molecular_functionferric iron binding
A0005506molecular_functioniron ion binding
A0006880biological_processintracellular sequestering of iron ion
A0006826biological_processiron ion transport
B0005737cellular_componentcytoplasm
B0008043cellular_componentintracellular ferritin complex
B0008199molecular_functionferric iron binding
B0005506molecular_functioniron ion binding
B0006880biological_processintracellular sequestering of iron ion
B0006826biological_processiron ion transport
C0005737cellular_componentcytoplasm
C0008043cellular_componentintracellular ferritin complex
C0008199molecular_functionferric iron binding
C0005506molecular_functioniron ion binding
C0006880biological_processintracellular sequestering of iron ion
C0006826biological_processiron ion transport
D0005737cellular_componentcytoplasm
D0008043cellular_componentintracellular ferritin complex
D0008199molecular_functionferric iron binding
D0005506molecular_functioniron ion binding
D0006880biological_processintracellular sequestering of iron ion
D0006826biological_processiron ion transport
E0005737cellular_componentcytoplasm
E0008043cellular_componentintracellular ferritin complex
E0008199molecular_functionferric iron binding
E0005506molecular_functioniron ion binding
E0006880biological_processintracellular sequestering of iron ion
E0006826biological_processiron ion transport
F0005737cellular_componentcytoplasm
F0008043cellular_componentintracellular ferritin complex
F0008199molecular_functionferric iron binding
F0005506molecular_functioniron ion binding
F0006880biological_processintracellular sequestering of iron ion
F0006826biological_processiron ion transport
G0005737cellular_componentcytoplasm
G0008043cellular_componentintracellular ferritin complex
G0008199molecular_functionferric iron binding
G0005506molecular_functioniron ion binding
G0006880biological_processintracellular sequestering of iron ion
G0006826biological_processiron ion transport
H0005737cellular_componentcytoplasm
H0008043cellular_componentintracellular ferritin complex
H0008199molecular_functionferric iron binding
H0005506molecular_functioniron ion binding
H0006880biological_processintracellular sequestering of iron ion
H0006826biological_processiron ion transport
I0005737cellular_componentcytoplasm
I0008043cellular_componentintracellular ferritin complex
I0008199molecular_functionferric iron binding
I0005506molecular_functioniron ion binding
I0006880biological_processintracellular sequestering of iron ion
I0006826biological_processiron ion transport
J0005737cellular_componentcytoplasm
J0008043cellular_componentintracellular ferritin complex
J0008199molecular_functionferric iron binding
J0005506molecular_functioniron ion binding
J0006880biological_processintracellular sequestering of iron ion
J0006826biological_processiron ion transport
K0005737cellular_componentcytoplasm
K0008043cellular_componentintracellular ferritin complex
K0008199molecular_functionferric iron binding
K0005506molecular_functioniron ion binding
K0006880biological_processintracellular sequestering of iron ion
K0006826biological_processiron ion transport
L0005737cellular_componentcytoplasm
L0008043cellular_componentintracellular ferritin complex
L0008199molecular_functionferric iron binding
L0005506molecular_functioniron ion binding
L0006880biological_processintracellular sequestering of iron ion
L0006826biological_processiron ion transport
M0005737cellular_componentcytoplasm
M0008043cellular_componentintracellular ferritin complex
M0008199molecular_functionferric iron binding
M0005506molecular_functioniron ion binding
M0006880biological_processintracellular sequestering of iron ion
M0006826biological_processiron ion transport
N0005737cellular_componentcytoplasm
N0008043cellular_componentintracellular ferritin complex
N0008199molecular_functionferric iron binding
N0005506molecular_functioniron ion binding
N0006880biological_processintracellular sequestering of iron ion
N0006826biological_processiron ion transport
O0005737cellular_componentcytoplasm
O0008043cellular_componentintracellular ferritin complex
O0008199molecular_functionferric iron binding
O0005506molecular_functioniron ion binding
O0006880biological_processintracellular sequestering of iron ion
O0006826biological_processiron ion transport
P0005737cellular_componentcytoplasm
P0008043cellular_componentintracellular ferritin complex
P0008199molecular_functionferric iron binding
P0005506molecular_functioniron ion binding
P0006880biological_processintracellular sequestering of iron ion
P0006826biological_processiron ion transport
Q0005737cellular_componentcytoplasm
Q0008043cellular_componentintracellular ferritin complex
Q0008199molecular_functionferric iron binding
Q0005506molecular_functioniron ion binding
Q0006880biological_processintracellular sequestering of iron ion
Q0006826biological_processiron ion transport
R0005737cellular_componentcytoplasm
R0008043cellular_componentintracellular ferritin complex
R0008199molecular_functionferric iron binding
R0005506molecular_functioniron ion binding
R0006880biological_processintracellular sequestering of iron ion
R0006826biological_processiron ion transport
S0005737cellular_componentcytoplasm
S0008043cellular_componentintracellular ferritin complex
S0008199molecular_functionferric iron binding
S0005506molecular_functioniron ion binding
S0006880biological_processintracellular sequestering of iron ion
S0006826biological_processiron ion transport
T0005737cellular_componentcytoplasm
T0008043cellular_componentintracellular ferritin complex
T0008199molecular_functionferric iron binding
T0005506molecular_functioniron ion binding
T0006880biological_processintracellular sequestering of iron ion
T0006826biological_processiron ion transport
U0005737cellular_componentcytoplasm
U0008043cellular_componentintracellular ferritin complex
U0008199molecular_functionferric iron binding
U0005506molecular_functioniron ion binding
U0006880biological_processintracellular sequestering of iron ion
U0006826biological_processiron ion transport
V0005737cellular_componentcytoplasm
V0008043cellular_componentintracellular ferritin complex
V0008199molecular_functionferric iron binding
V0005506molecular_functioniron ion binding
V0006880biological_processintracellular sequestering of iron ion
V0006826biological_processiron ion transport
W0005737cellular_componentcytoplasm
W0008043cellular_componentintracellular ferritin complex
W0008199molecular_functionferric iron binding
W0005506molecular_functioniron ion binding
W0006880biological_processintracellular sequestering of iron ion
W0006826biological_processiron ion transport
X0005737cellular_componentcytoplasm
X0008043cellular_componentintracellular ferritin complex
X0008199molecular_functionferric iron binding
X0005506molecular_functioniron ion binding
X0006880biological_processintracellular sequestering of iron ion
X0006826biological_processiron ion transport
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Functional Information from PDB Data

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Functional Information from PDB atom coordinates for the "HETATM" binding sites

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Functional Information from PROSITE/UniProt

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Functional Information from SwissProt/UniProt

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Catalytic Information from CSA

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> Sequence Neighbor

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