4V1M

Architecture of the RNA polymerase II-Mediator core transcription initiation complex

Summary for 4V1M

• Entry DOI: 10.2210/pdb4v1m/pdb
• Related: 4V1N 4V1O
• EMDB information: 2784
• Descriptor: DNA-DIRECTED RNA POLYMERASE II SUBUNIT RPB1, DNA-DIRECTED RNA POLYMERASES I, II, AND III SUBUNIT RPABC 4, 5'-D(*AP*AP*GP*TP*AP*CP*TP*TP*GP*AP)-3', ... (15 entities in total)
• Functional Keywords: transcription, transcription initiation, rna polymerase ii, general transcription factors
• Biological source: SACCHAROMYCES CEREVISIAE (BAKER'S YEAST); More
• Cellular location: Nucleus: P04050 P08518 P16370 P20434 P20436 P38902; Nucleus, nucleolus : P40422 P27999 P22139; Cytoplasm : P20435
• Total number of polymer chains: 13
• Total formula weight: 481286.62

Authors

Plaschka, C.,Lariviere, L.,Wenzeck, L.,Hemann, M.,Tegunov, D.,Petrotchenko, E.V.,Borchers, C.H.,Baumeister, W.,Herzog, F.,Villa, E.,Cramer, P. (deposition date: 2014-09-29, release date: 2015-02-04, Last modification date: 2024-05-08)

Primary citation

Plaschka, C.,Lariviere, L.,Wenzeck, L.,Seizl, M.,Hemann, M.,Tegunov, D.,Petrotchenko, E.V.,Borchers, C.H.,Baumeister, W.,Herzog, F.,Villa, E.,Cramer, P.
Architecture of the RNA Polymerase II-Mediator Core Initiation Complex.
Nature, 518:376-, 2015

PubMed Abstract: The conserved co-activator complex Mediator enables regulated transcription initiation by RNA polymerase (Pol) II. Here we reconstitute an active 15-subunit core Mediator (cMed) comprising all essential Mediator subunits from Saccharomyces cerevisiae. The cryo-electron microscopic structure of cMed bound to a core initiation complex was determined at 9.7 Å resolution. cMed binds Pol II around the Rpb4-Rpb7 stalk near the carboxy-terminal domain (CTD). The Mediator head module binds the Pol II dock and the TFIIB ribbon and stabilizes the initiation complex. The Mediator middle module extends to the Pol II foot with a 'plank' that may influence polymerase conformation. The Mediator subunit Med14 forms a 'beam' between the head and middle modules and connects to the tail module that is predicted to bind transcription activators located on upstream DNA. The Mediator 'arm' and 'hook' domains contribute to a 'cradle' that may position the CTD and TFIIH kinase to stimulate Pol II phosphorylation.

PubMed: 25652824
DOI: 10.1038/NATURE14229

Experimental method

ELECTRON MICROSCOPY (6.6 Å)