4V16

KlHsv2 with loop 6CD replaced by a Gly-Ser linker

Summary for 4V16

• Entry DOI: 10.2210/pdb4v16/pdb
• Descriptor: SVP1-LIKE PROTEIN 2 (2 entities in total)
• Functional Keywords: protein transport, proppin, phosphoinositide binding
• Biological source: KLUYVEROMYCES LACTIS
• Cellular location: Vacuole membrane ; Peripheral membrane protein : Q6CN23
• Total number of polymer chains: 1
• Total formula weight: 37563.87

Authors

Busse, R.A.,Scacioc, A.,Krick, R.,Perez-Lara, A.,Thumm, M.,Kuhnel, K. (deposition date: 2014-09-25, release date: 2015-04-01, Last modification date: 2024-01-10)

Primary citation

Busse, R.A.,Scacioc, A.,Krick, R.,Perez-Lara, A.,Thumm, M.,Kuhnel, K.
Characterization of Proppin-Phosphoinositide Binding and Role of Loop 6Cd in Proppin-Membrane Binding.
Biophys.J., 108:2223-, 2015

PubMed Abstract: PROPPINs (β-propellers that bind polyphosphoinositides) are a family of PtdIns3P- and PtdIns(3,5)P2-binding proteins that play an important role in autophagy. We analyzed PROPPIN-membrane binding through isothermal titration calorimetry (ITC), stopped-flow measurements, mutagenesis studies, and molecular dynamics (MD) simulations. ITC measurements showed that the yeast PROPPIN family members Atg18, Atg21, and Hsv2 bind PtdIns3P and PtdIns(3,5)P2 with high affinities in the nanomolar to low-micromolar range and have two phosphoinositide (PIP)-binding sites. Single PIP-binding site mutants have a 15- to 30-fold reduced affinity, which explains the requirement of two PIP-binding sites in PROPPINs. Hsv2 bound small unilamellar vesicles with a higher affinity than it bound large unilamellar vesicles in stopped-flow measurements. Thus, we conclude that PROPPIN membrane binding is curvature dependent. MD simulations revealed that loop 6CD is an anchor for membrane binding, as it is the region of the protein that inserts most deeply into the lipid bilayer. Mutagenesis studies showed that both hydrophobic and electrostatic interactions are required for membrane insertion of loop 6CD. We propose a model for PROPPIN-membrane binding in which PROPPINs are initially targeted to membranes through nonspecific electrostatic interactions and are then retained at the membrane through PIP binding.

PubMed: 25954880
DOI: 10.1016/J.BPJ.2015.03.045

Experimental method

X-RAY DIFFRACTION (2.8 Å)