4V16
KlHsv2 with loop 6CD replaced by a Gly-Ser linker
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2011-02-04 |
| Detector | DECTRIS PILATUS |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 101.700, 101.700, 78.030 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 42.602 - 2.800 |
| R-factor | 0.2004 |
| Rwork | 0.198 |
| R-free | 0.23930 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4av9 |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.757 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | MOLREP |
| Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | 2.900 |
| High resolution limit [Å] | 2.800 | 2.800 |
| Rmerge | 0.080 | 0.440 |
| Number of reflections | 11779 | |
| <I/σ(I)> | 19.9 | 4 |
| Completeness [%] | 99.0 | 99 |
| Redundancy | 5.5 | 5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 14 % PEG 3350, 0.2 M MGCL2, 0.1 M TRIS PH 9.0 |
