4UWE

Structure of the ryanodine receptor at resolution of 8.5 A in partially open state

Summary for 4UWE

• Entry DOI: 10.2210/pdb4uwe/pdb
• Related: 4UWA
• EMDB information: 2752
• Descriptor: RYANODINE RECEPTOR 1 (1 entity in total)
• Functional Keywords: signaling protein, calcium binding, ion channel, muscular contraction, conformational changes.
• Biological source: ORYCTOLAGUS CUNICULUS (RABBIT)
• Total number of polymer chains: 4
• Total formula weight: 1894515.62

Authors

Efremov, R.G.,Leitner, A.,Aebersold, R.,Raunser, S. (deposition date: 2014-08-11, release date: 2014-12-10, Last modification date: 2024-05-08)

Primary citation

Efremov, R.G.,Leitner, A.,Aebersold, R.,Raunser, S.
Architecture and Conformational Switch Mechanism of the Ryanodine Receptor.
Nature, 517:39-, 2015

PubMed Abstract: Muscle contraction is initiated by the release of calcium (Ca(2+)) from the sarcoplasmic reticulum into the cytoplasm of myocytes through ryanodine receptors (RyRs). RyRs are homotetrameric channels with a molecular mass of more than 2.2 megadaltons that are regulated by several factors, including ions, small molecules and proteins. Numerous mutations in RyRs have been associated with human diseases. The molecular mechanism underlying the complex regulation of RyRs is poorly understood. Using electron cryomicroscopy, here we determine the architecture of rabbit RyR1 at a resolution of 6.1 Å. We show that the cytoplasmic moiety of RyR1 contains two large α-solenoid domains and several smaller domains, with folds suggestive of participation in protein-protein interactions. The transmembrane domain represents a chimaera of voltage-gated sodium and pH-activated ion channels. We identify the calcium-binding EF-hand domain and show that it functions as a conformational switch allosterically gating the channel.

PubMed: 25470059
DOI: 10.1038/NATURE13916

Experimental method

ELECTRON MICROSCOPY (8.5 Å)