4UWE
Structure of the ryanodine receptor at resolution of 8.5 A in partially open state
EM specimen
| Vitrification | Yes |
| Instrument | GATAN CRYOPLUNGE 3 |
EM Data Collection
| Microscope | JEOL 3200FSC |
| Electron Gun | FIELD EMISSION GUN |
| Accelerating Voltage (kV) | 200 |
| Cs (mm) | 4.1 |
| Detector | TVIPS TEMCAM-F816 (8k x 8k) |
| Number of Images | 1041 |
3D Reconstruction
| Method | SINGLE PARTICLE |
| Resolution (Å) | 8.5 |
| Software | UCSF Chimera,SPARX |
| Symmetry | C4 |
