4UIH

Cryo-EM structure of Dengue virus serotype 2 strain New Guinea-C complexed with human antibody 2D22 Fab at 37 degree C. The Fab molecules were added to the virus before 37 degree C incubation.

Summary for 4UIH

• Entry DOI: 10.2210/pdb4uih/pdb
• Related: 4UIF
• EMDB information: 2968
• Descriptor: DENGUE VIRUS SEROTYPE 2 STRAIN NEW GUINEA-C E PROTEIN ECTODOMAIN, ANTIGEN-BINDING FRAGMENT OF HUMAN ANTIBODY 2D22 -HEAVY CHAIN, ANTIGEN-BINDING FRAGMENT OF HUMAN ANTIBODY 2D22 -LIGHT CHAIN (3 entities in total)
• Functional Keywords: viral protein, dengue virus, dengue serotype 2, human antibody, cryo-em, neutralization
• Biological source: DENGUE VIRUS 2 (ASIAN TIGER MOSQUITO); More
• Cellular location: Capsid protein C: Virion . Peptide pr: Secreted . Small envelope protein M: Virion membrane ; Multi-pass membrane protein . Envelope protein E: Virion membrane ; Multi-pass membrane protein . Non-structural protein 1: Secreted . Non-structural protein 2A: Host endoplasmic reticulum membrane ; Multi-pass membrane protein . Serine protease subunit NS2B: Host endoplasmic reticulum membrane; Multi-pass membrane protein . Serine protease NS3: Host endoplasmic reticulum membrane ; Peripheral membrane protein ; Cytoplasmic side . Non-structural protein 4A: Host endoplasmic reticulum membrane ; Multi-pass membrane protein . Non-structural protein 4B: Host endoplasmic reticulum membrane ; Multi-pass membrane protein . RNA-directed RNA polymerase NS5: Host endoplasmic reticulum membrane; Peripheral membrane protein; Cytoplasmic side: P14340
• Total number of polymer chains: 7
• Total formula weight: 214882.73

Authors

Fibriansah, G.,Ibarra, K.D.,Ng, T.-S.,Smith, S.A.,Tan, J.L.,Lim, X.N.,Ooi, J.S.G.,Kostyuchenko, V.A.,Wang, J.,de Silva, A.M.,Harris, E.,Crowe, J.E.,Lok, S.-M. (deposition date: 2015-03-30, release date: 2015-07-15, Last modification date: 2018-02-07)

• Primary citation: Fibriansah, G.,Ibarra, K.D.,Ng, T.S.,Smith, S.A.,Tan, J.L.,Lim, X.N.,Ooi, J.S.,Kostyuchenko, V.A.,Wang, J.,de Silva, A.M.,Harris, E.,Crowe, J.E.,Lok, S.M.; Cryo-EM structure of an antibody that neutralizes dengue virus type 2 by locking E protein dimers.; Science, 349:88-91, 2015; PubMed: 26138979; DOI: 10.1126/science.aaa8651

Experimental method

ELECTRON MICROSCOPY (20 Å)