4UFC

Crystal structure of the GH95 enzyme BACOVA_03438

Summary for 4UFC

• Entry DOI: 10.2210/pdb4ufc/pdb
• Descriptor: GH95, CALCIUM ION, beta-L-galactopyranose, ... (4 entities in total)
• Functional Keywords: hydrolase, glycoside hydrolase, xylan, alpha-l-galactosidase, gut microbiota
• Biological source: BACTEROIDES OVATUS
• Total number of polymer chains: 2
• Total formula weight: 183845.09

Authors

Rogowski, A.,Briggs, J.A.,Mortimer, J.C.,Tryfona, T.,Terrapon, N.,Lowe, E.C.,Basle, A.,Morland, C.,Day, A.M.,Zheng, H.,Rogers, T.E.,Thompson, P.,Hawkins, A.R.,Yadav, M.P.,Henrissat, B.,Martens, E.C.,Dupree, P.,Gilbert, H.J.,Bolam, D.N. (deposition date: 2015-03-16, release date: 2015-07-08, Last modification date: 2023-12-20)

Primary citation

Rogowski, A.,Briggs, J.A.,Mortimer, J.C.,Tryfona, T.,Terrapon, N.,Lowe, E.C.,Basle, A.,Morland, C.,Day, A.M.,Zheng, H.,Rogers, T.E.,Thompson, P.,Hawkins, A.R.,Yadav, M.P.,Henrissat, B.,Martens, E.C.,Dupree, P.,Gilbert, H.J.,Bolam, D.N.
Glycan Complexity Dictates Microbial Resource Allocation in the Large Intestine.
Nat.Commun., 6:7481-, 2015

PubMed Abstract: The structure of the human gut microbiota is controlled primarily through the degradation of complex dietary carbohydrates, but the extent to which carbohydrate breakdown products are shared between members of the microbiota is unclear. We show here, using xylan as a model, that sharing the breakdown products of complex carbohydrates by key members of the microbiota, such as Bacteroides ovatus, is dependent on the complexity of the target glycan. Characterization of the extensive xylan degrading apparatus expressed by B. ovatus reveals that the breakdown of the polysaccharide by the human gut microbiota is significantly more complex than previous models suggested, which were based on the deconstruction of xylans containing limited monosaccharide side chains. Our report presents a highly complex and dynamic xylan degrading apparatus that is fine-tuned to recognize the different forms of the polysaccharide presented to the human gut microbiota.

PubMed: 26112186
DOI: 10.1038/NCOMMS8481

Experimental method

X-RAY DIFFRACTION (2.81 Å)