4U2M

Crystal structure of a complex of the Miz1- and BCL6 POZ domains.

Summary for 4U2M

• Entry DOI: 10.2210/pdb4u2m/pdb
• Related: 4u2n
• Descriptor: Zinc finger and BTB domain-containing protein 17,B-cell lymphoma 6 protein (2 entities in total)
• Functional Keywords: poz domain, btb domain, transcription
• Biological source: Homo sapiens (Human); More
• Cellular location: Nucleus : P41182
• Total number of polymer chains: 4
• Total formula weight: 113310.59

Authors

Stead, M.A.,Wright, S.C. (deposition date: 2014-07-17, release date: 2014-12-10, Last modification date: 2024-05-08)

Primary citation

Stead, M.A.,Wright, S.C.
Structures of heterodimeric POZ domains of Miz1/BCL6 and Miz1/NAC1.
Acta Crystallogr.,Sect.F, 70:1591-1596, 2014

PubMed Abstract: The POZ domain is an evolutionarily conserved protein-protein interaction domain that is found in approximately 40 mammalian transcription factors. POZ domains mediate both homodimerization and the heteromeric interactions of different POZ-domain transcription factors with each other. Miz1 is a POZ-domain transcription factor that regulates cell-cycle arrest and DNA-damage responses. The activities of Miz1 are altered by its interaction with the POZ-domain transcriptional repressors BCL6 and NAC1, and these interactions have been implicated in tumourigenesis in B-cell lymphomas and in ovarian serous carcinomas that overexpress BCL6 and NAC1, respectively. A strategy for the purification of tethered POZ domains that form forced heterodimers is described, and crystal structures of the heterodimeric POZ domains of Miz1/BCL6 and of Miz1/NAC1 are reported. These structures will be relevant for the design of therapeutics that target POZ-domain interaction interfaces.

PubMed: 25484205
DOI: 10.1107/S2053230X14023449

Experimental method

X-RAY DIFFRACTION (2.23 Å)