4U2M

Crystal structure of a complex of the Miz1- and BCL6 POZ domains.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I04
Synchrotron site	Diamond
Beamline	I04
Temperature [K]	100
Detector technology	PIXEL
Collection date	2014-04-25
Detector	PSI PILATUS 6M
Wavelength(s)	0.97949
Spacegroup name	P 21 21 21
Unit cell lengths	91.850, 97.880, 119.880
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	58.470 - 2.230
R-factor	0.2129
Rwork	0.211
R-free	0.24040
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.015
RMSD bond angle	1.502
Data reduction software	XDS
Data scaling software	Aimless (0.2.17)
Phasing software	PHASER
Refinement software	REFMAC (5.8.0073)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	58.470	58.470	2.290
High resolution limit [Å]	2.230	9.970	2.230
Rmerge	0.046	0.021	0.311
Rpim	0.025	0.013	0.173
Total number of observations	215105	2304	14633
Number of reflections	52962
<I/σ(I)>	19	50.8	4.8
Completeness [%]	99.3	98.1	96.2
Redundancy	4.1	3.4	3.9
CC(1/2)	0.997	0.990	0.919

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	4.5	291	4 % PEG 3350, 3 % 2-propanol, 0.2 M ammonium citrate pH 4.5