4TWS

Gadolinium Derivative of Tetragonal Hen Egg-White Lysozyme at 1.45 A Resolution

Summary for 4TWS

• Entry DOI: 10.2210/pdb4tws/pdb
• Descriptor: Lysozyme C, GADOLINIUM ATOM, 10-((2R)-2-HYDROXYPROPYL)-1,4,7,10-TETRAAZACYCLODODECANE 1,4,7-TRIACETIC ACID, ... (6 entities in total)
• Functional Keywords: lysozyme, gadolinium, hydrolase
• Biological source: Gallus gallus (Chicken)
• Total number of polymer chains: 1
• Total formula weight: 16592.13

Authors

Holton, J.M.,Classen, S.,Frankel, K.A.,Tainer, J.A. (deposition date: 2014-07-01, release date: 2014-08-20, Last modification date: 2024-11-20)

Primary citation

Holton, J.M.,Classen, S.,Frankel, K.A.,Tainer, J.A.
The R-factor gap in macromolecular crystallography: an untapped potential for insights on accurate structures.
Febs J., 281:4046-4060, 2014

PubMed Abstract: In macromolecular crystallography, the agreement between observed and predicted structure factors (Rcryst and Rfree ) is seldom better than 20%. This is much larger than the estimate of experimental error (Rmerge ). The difference between Rcryst and Rmerge is the R-factor gap. There is no such gap in small-molecule crystallography, for which calculated structure factors are generally considered more accurate than the experimental measurements. Perhaps the true noise level of macromolecular data is higher than expected? Or is the gap caused by inaccurate phases that trap refined models in local minima? By generating simulated diffraction patterns using the program MLFSOM, and including every conceivable source of experimental error, we show that neither is the case. Processing our simulated data yielded values that were indistinguishable from those of real data for all crystallographic statistics except the final Rcryst and Rfree . These values decreased to 3.8% and 5.5% for simulated data, suggesting that the reason for high R-factors in macromolecular crystallography is neither experimental error nor phase bias, but rather an underlying inadequacy in the models used to explain our observations. The present inability to accurately represent the entire macromolecule with both its flexibility and its protein-solvent interface may be improved by synergies between small-angle X-ray scattering, computational chemistry and crystallography. The exciting implication of our finding is that macromolecular data contain substantial hidden and untapped potential to resolve ambiguities in the true nature of the nanoscale, a task that the second century of crystallography promises to fulfill.

PubMed: 25040949
DOI: 10.1111/febs.12922

Experimental method

X-RAY DIFFRACTION (1.45 Å)