4TVX
Crystal structure of the E. coli CRISPR RNA-guided surveillance complex, Cascade
This is a non-PDB format compatible entry.
Summary for 4TVX
| Entry DOI | 10.2210/pdb4tvx/pdb |
| Descriptor | CRISPR system Cascade subunit CasB, CRISPR system Cascade subunit CasC, CRISPR system Cascade subunit CasE, ... (7 entities in total) |
| Functional Keywords | crispr, rna, surveillance, complex, hydrolase-rna complex, hydrolase/rna |
| Biological source | Escherichia coli More |
| Total number of polymer chains | 24 |
| Total formula weight | 804324.65 |
| Authors | Jackson, R.N.,Golden, S.M.,Carter, J.,Wiedenheft, B. (deposition date: 2014-06-28, release date: 2014-08-13, Last modification date: 2024-10-23) |
| Primary citation | Jackson, R.N.,Golden, S.M.,van Erp, P.B.,Carter, J.,Westra, E.R.,Brouns, S.J.,van der Oost, J.,Terwilliger, T.C.,Read, R.J.,Wiedenheft, B. Structural biology. Crystal structure of the CRISPR RNA-guided surveillance complex from Escherichia coli. Science, 345:1473-1479, 2014 Cited by PubMed Abstract: Clustered regularly interspaced short palindromic repeats (CRISPRs) are essential components of RNA-guided adaptive immune systems that protect bacteria and archaea from viruses and plasmids. In Escherichia coli, short CRISPR-derived RNAs (crRNAs) assemble into a 405-kilodalton multisubunit surveillance complex called Cascade (CRISPR-associated complex for antiviral defense). Here we present the 3.24 angstrom resolution x-ray crystal structure of Cascade. Eleven proteins and a 61-nucleotide crRNA assemble into a seahorse-shaped architecture that binds double-stranded DNA targets complementary to the crRNA-guide sequence. Conserved sequences on the 3' and 5' ends of the crRNA are anchored by proteins at opposite ends of the complex, whereas the guide sequence is displayed along a helical assembly of six interwoven subunits that present five-nucleotide segments of the crRNA in pseudo-A-form configuration. The structure of Cascade suggests a mechanism for assembly and provides insights into the mechanisms of target recognition. PubMed: 25103409DOI: 10.1126/science.1256328 PDB entries with the same primary citation |
| Experimental method | X-RAY DIFFRACTION (3.24 Å) |
Structure validation
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