4TVX

Crystal structure of the E. coli CRISPR RNA-guided surveillance complex, Cascade

This is a non-PDB format compatible entry.

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B, C, D (W, V, K, J)	CRISPR system Cascade subunit CasB	polymer	165	19231.0	4	UniProt (P76632) Pfam (PF09485)	Escherichia coli
2	E, F, G, H, I... (F, D, E, C, B...)	CRISPR system Cascade subunit CasC	polymer	363	40071.4	12	UniProt (Q46899) Pfam (PF09344)	Escherichia coli
3	K, T (A, M)	CRISPR system Cascade subunit CasE	polymer	199	22324.0	2	UniProt (Q46897) Pfam (PF08798)	Escherichia coli	CasE endoRNase,crRNA endonuclease
4	L, S (H, T)	CRISPR system Cascade subunit CasD	polymer	224	25239.8	2	UniProt (Q46898) Pfam (PF09704)	Escherichia coli
5	U, V (L, X)	Escherichia coli strain ECOR44 cluster 1 CRISPR region	polymer	61	19677.6	2	GenBank (50811866)	Escherichia coli
6	W, X (U, I)	CRISPR system Cascade subunit CasA	polymer	502	55965.0	2	UniProt (Q46901) Pfam (PF09481)	Escherichia coli	CRISPR type I-E/Ecoli-associated protein CasA/Cse1,CRISPR-associated protein CasA/Cse1
7	Y, Z (U, I)	ZINC ION	non-polymer		65.4	2	Chemie (ZN)

Sequence modifications

W, V, K, J: 1 - 160 (UniProt: P76632)

PDB	External Database	Details
Gly -4	-	expression tag
Pro -3	-	expression tag
Gly -2	-	expression tag
Tyr -1	-	expression tag
Gln 0	-	expression tag

L, X: 1 - 61 (GenBank: 50811866)

PDB	External Database	Details
A 42	C 454	conflict
C 53	U 443	conflict

Contents of the asymmetric unit

Polymers	Number of chains	24
	Total formula weight	804193.8
Non-Polymers*	Number of molecules	2
	Total formula weight	130.8
All*	Total formula weight	804324.7

*Water molecules are not included.