4RZT

Lac repressor engineered to bind sucralose, sucralose-bound tetramer

Summary for 4RZT

• Entry DOI: 10.2210/pdb4rzt/pdb
• Related: 4RZS
• Related PRD ID: PRD_900090
• Descriptor: Lac repressor, 4-chloro-4-deoxy-alpha-D-galactopyranose-(1-2)-1,6-dichloro-1,6-dideoxy-beta-D-fructofuranose (3 entities in total)
• Functional Keywords: sucralose, laci, lac repressor, lactose operon repressor, protein design, allostery, escherichia coli, transcription
• Biological source: Escherichia coli
• Total number of polymer chains: 4
• Total formula weight: 165124.49

Authors

Arbing, M.A.,Cascio, D.,Sawaya, M.R.,Kosuri, S.,Church, G.M. (deposition date: 2014-12-24, release date: 2015-12-23, Last modification date: 2024-02-28)

• Primary citation: Taylor, N.D.,Garruss, A.S.,Moretti, R.,Chan, S.,Arbing, M.A.,Cascio, D.,Rogers, J.K.,Isaacs, F.J.,Kosuri, S.,Baker, D.,Fields, S.,Church, G.M.,Raman, S.; Engineering an allosteric transcription factor to respond to new ligands.; Nat.Methods, 13:177-183, 2016; PubMed: 26689263; DOI: 10.1038/nmeth.3696

Experimental method

X-RAY DIFFRACTION (3.1 Å)