4RZT
Lac repressor engineered to bind sucralose, sucralose-bound tetramer
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-C |
| Synchrotron site | APS |
| Beamline | 24-ID-C |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2014-10-16 |
| Detector | DECTRIS PILATUS 6M-F |
| Wavelength(s) | 0.9791 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 163.590, 74.080, 149.160 |
| Unit cell angles | 90.00, 119.91, 90.00 |
Refinement procedure
| Resolution | 81.660 - 3.100 |
| R-factor | 0.20713 |
| Rwork | 0.205 |
| R-free | 0.24335 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.496 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0049) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 81.670 | 3.180 | |
| High resolution limit [Å] | 3.030 | 13.880 | 3.030 |
| Rmerge | 0.079 | 0.025 | 0.580 |
| Number of reflections | 24030 | ||
| <I/σ(I)> | 12.24 | 38.32 | 2.04 |
| Completeness [%] | 84.8 | 93.5 | 11.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.4 | 298 | 0.1M HEPES, pH7.5, 10% polyethylene glycol 6000, and 5% MPD, pH 7.4, vapor diffusion, hanging drop, temperature 298K |
