4RZF

Crystal Structure Analysis of the NUR77 Ligand Binding Domain, S441W mutant

Summary for 4RZF

• Entry DOI: 10.2210/pdb4rzf/pdb
• Related: 4RZE 4RZG
• Descriptor: Nuclear receptor subfamily 4 group A member 1, GLYCEROL (3 entities in total)
• Functional Keywords: transcription
• Biological source: Homo sapiens (human)
• Cellular location: Cytoplasm: P22736
• Total number of polymer chains: 2
• Total formula weight: 57702.77

Authors

Li, F.,Tian, X.,Li, A.,Li, L.,Liu, Y.,Chen, H.,Wu, Q.,Lin, T. (deposition date: 2014-12-21, release date: 2015-03-18, Last modification date: 2024-02-28)

Primary citation

Li, L.,Liu, Y.,Chen, H.Z.,Li, F.W.,Wu, J.F.,Zhang, H.K.,He, J.P.,Xing, Y.Z.,Chen, Y.,Wang, W.J.,Tian, X.Y.,Li, A.Z.,Zhang, Q.,Huang, P.Q.,Han, J.,Lin, T.,Wu, Q.
Impeding the interaction between Nur77 and p38 reduces LPS-induced inflammation.
Nat.Chem.Biol., 11:339-346, 2015

PubMed Abstract: Sepsis, a hyperinflammatory response that can result in multiple organ dysfunctions, is a leading cause of mortality from infection. Here, we show that orphan nuclear receptor Nur77 (also known as TR3) can enhance resistance to lipopolysaccharide (LPS)-induced sepsis in mice by inhibiting NF-κB activity and suppressing aberrant cytokine production. Nur77 directly associates with p65 to block its binding to the κB element. However, this function of Nur77 is countered by the LPS-activated p38α phosphorylation of Nur77. Dampening the interaction between Nur77 and p38α would favor Nur77 suppression of the hyperinflammatory response. A compound, n-pentyl 2-[3,5-dihydroxy-2-(1-nonanoyl) phenyl]acetate, screened from a Nur77-biased library, blocked the Nur77-p38α interaction by targeting the ligand-binding domain of Nur77 and restored the suppression of the hyperinflammatory response through Nur77 inhibition of NF-κB. This study associates the nuclear receptor with immune homeostasis and implicates a new therapeutic strategy to treat hyperinflammatory responses by targeting a p38α substrate to modulate p38α-regulated functions.

PubMed: 25822914
DOI: 10.1038/nchembio.1788

Experimental method

X-RAY DIFFRACTION (1.99 Å)