4RUB

A CRYSTAL FORM OF RIBULOSE-1,5-BISPHOSPHATE CARBOXYLASE(SLASH)OXYGENASE FROM NICOTIANA TABACUM IN THE ACTIVATED STATE

Summary for 4RUB

• Entry DOI: 10.2210/pdb4rub/pdb
• Descriptor: RIBULOSE 1,5-BISPHOSPHATE CARBOXYLASE/OXYGENASE (FORM IV), MAGNESIUM ION, 2-CARBOXYARABINITOL-1,5-DIPHOSPHATE, ... (5 entities in total)
• Functional Keywords: lyase(carbon-carbon)
• Biological source: Nicotiana tabacum (common tobacco); More
• Cellular location: Plastid, chloroplast: P00876 P69249
• Total number of polymer chains: 8
• Total formula weight: 271580.24

Authors

Schreuder, H.,Cascio, D.,Curmi, P.M.G.,Eisenberg, D. (deposition date: 1990-05-25, release date: 1992-10-15, Last modification date: 2024-06-05)

Primary citation

Suh, S.W.,Cascio, D.,Chapman, M.S.,Eisenberg, D.
A crystal form of ribulose-1,5-bisphosphate carboxylase/oxygenase from Nicotiana tabacum in the activated state.
J.Mol.Biol., 197:363-365, 1987

PubMed Abstract: A new crystal form of ribulose-1,5-bisphosphate carboxylase/oxygenase (EC 4.1.1.39) from Nicotiana tabacum has been obtained at alkaline pH with polyethylene glycol 8000 in the presence of a non-ionic detergent, beta-octyl glucoside. The crystals are grown at room temperature by the hanging-drop vapor diffusion technique from a protein solution containing enzyme complexed with CO2, Mg2+, and the transition state analog 2-C-carboxy-D-arabinitol-1,5-bisphosphate. The crystals belong to the the space group P3(1)21 (or P3(2)21) with the cell parameters a = 204.6 A, and c = 117.4 A (1 A = 0.1 nm). The asymmetric unit contains half (L4S4: L, large subunit, 53,000 Mr; S, small subunit, 15,000 Mr) of a hexadecameric molecule (L8S8, 540,000 Mr). The crystals diffract to at least 2.6 A Bragg spacing and are suitable for X-ray structure determination.

PubMed: 3681999
DOI: 10.1016/0022-2836(87)90129-X

Experimental method

X-RAY DIFFRACTION (2.7 Å)