4RUB
A CRYSTAL FORM OF RIBULOSE-1,5-BISPHOSPHATE CARBOXYLASE(SLASH)OXYGENASE FROM NICOTIANA TABACUM IN THE ACTIVATED STATE
Experimental procedure
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 204.600, 204.600, 117.400 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | ? - 2.700 |
| R-factor | 0.202 |
| RMSD bond length | 0.019 |
| RMSD bond angle | 3.200 |
| Refinement software | PROFFT |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 8.3 * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | enzyme | 14.8 (mg/ml) | |
| 10 | 1 | reservoir | Tris-HCl | 50 (mM) | |
| 11 | 1 | reservoir | 50 (mM) | ||
| 12 | 1 | reservoir | 10 (mM) | ||
| 13 | 1 | reservoir | 20 (mM) | ||
| 14 | 1 | reservoir | 1 (mM) | ||
| 2 | 1 | drop | Tris-HCl | 33.8 (mM) | |
| 3 | 1 | drop | 47.5 (mM) | ||
| 4 | 1 | drop | 9.5 (mM) | ||
| 5 | 1 | drop | 18 (mM) | ||
| 6 | 1 | drop | 0.95 (mM) | ||
| 7 | 1 | drop | PEG8000 | 4.9 (%(w/v)) | |
| 8 | 1 | drop | beta-octyl glucoside | 0.5 (%(w/v)) | |
| 9 | 1 | reservoir | PEG8000 | 12.8-13.0 (%(w/v)) |
