4RIB

FAN1 Nuclease bound to 5' phosphorylated p(dT) single flap DNA

4RIB の概要

• エントリーDOI: 10.2210/pdb4rib/pdb
• 関連するPDBエントリー: 4RI8 4RI9 4RIA 4RIC 4RID
• 分子名称: Fanconi-associated nuclease 1, DNA (5'-D(P*TP*AP*GP*CP*CP*AP*CP*GP*CP*CP*TP*AP*GP*AP*CP*TP*CP*CP*TP*C)-3'), DNA (5'-D(*GP*CP*TP*GP*AP*GP*GP*AP*GP*TP*CP*T)-3'), ... (5 entities in total)
• 機能のキーワード: nuclease, hydrolase-dna complex, hydrolase/dna
• 由来する生物種: Homo sapiens (human)
• 細胞内の位置: Nucleus : Q9Y2M0
• タンパク質・核酸の鎖数: 8
• 化学式量合計: 176064.46

構造登録者

Pavletich, N.P.,Wang, R. (登録日: 2014-10-05, 公開日: 2014-12-03, 最終更新日: 2024-02-28)

主引用文献

Wang, R.,Persky, N.S.,Yoo, B.,Ouerfelli, O.,Smogorzewska, A.,Elledge, S.J.,Pavletich, N.P.
DNA repair. Mechanism of DNA interstrand cross-link processing by repair nuclease FAN1.
Science, 346:1127-1130, 2014

PubMed Abstract: DNA interstrand cross-links (ICLs) are highly toxic lesions associated with cancer and degenerative diseases. ICLs can be repaired by the Fanconi anemia (FA) pathway and through FA-independent processes involving the FAN1 nuclease. In this work, FAN1-DNA crystal structures and biochemical data reveal that human FAN1 cleaves DNA successively at every third nucleotide. In vitro, this exonuclease mechanism allows FAN1 to excise an ICL from one strand through flanking incisions. DNA access requires a 5'-terminal phosphate anchor at a nick or a 1- or 2-nucleotide flap and is augmented by a 3' flap, suggesting that FAN1 action is coupled to DNA synthesis or recombination. FAN1's mechanism of ICL excision is well suited for processing other localized DNA adducts as well.

PubMed: 25430771
DOI: 10.1126/science.1258973

実験手法

X-RAY DIFFRACTION (3.25 Å)