4RDQ

Calcium-activated chloride channel bestrophin-1, from chicken, in complex with Fab antibody fragments, chloride and calcium

4RDQ の概要

• エントリーDOI: 10.2210/pdb4rdq/pdb
• 分子名称: Bestrophin-1, Fab antibody fragment, light chain, Fab antibody fragment, heavy chain, ... (9 entities in total)
• 機能のキーワード: transmembrane protein, membrane protein, ion channel, calcium-activated chloride channel, cacc, anion channel, membrane, transport protein
• 由来する生物種: Gallus gallus (bantam,chickens); 詳細
• タンパク質・核酸の鎖数: 15
• 化学式量合計: 479356.81

構造登録者

Dickson, V.K.,Pedi, L.,Long, S.B. (登録日: 2014-09-19, 公開日: 2014-10-22, 最終更新日: 2024-11-20)

主引用文献

Kane Dickson, V.,Pedi, L.,Long, S.B.
Structure and insights into the function of a Ca(2+)-activated Cl(-) channel.
Nature, 516:213-218, 2014

PubMed Abstract: Bestrophin calcium-activated chloride channels (CaCCs) regulate the flow of chloride and other monovalent anions across cellular membranes in response to intracellular calcium (Ca(2+)) levels. Mutations in bestrophin 1 (BEST1) cause certain eye diseases. Here we present X-ray structures of chicken BEST1-Fab complexes, at 2.85 Å resolution, with permeant anions and Ca(2+). Representing, to our knowledge, the first structure of a CaCC, the eukaryotic BEST1 channel, which recapitulates CaCC function in liposomes, is formed from a pentameric assembly of subunits. Ca(2+) binds to the channel's large cytosolic region. A single ion pore, approximately 95 Å in length, is located along the central axis and contains at least 15 binding sites for anions. A hydrophobic neck within the pore probably forms the gate. Phenylalanine residues within it may coordinate permeating anions via anion-π interactions. Conformational changes observed near the 'Ca(2+) clasp' hint at the mechanism of Ca(2+)-dependent gating. Disease-causing mutations are prevalent within the gating apparatus.

PubMed: 25337878
DOI: 10.1038/nature13913

実験手法

X-RAY DIFFRACTION (2.85 Å)