4QT8

Crystal Structure of RON Sema-PSI-IPT1 extracellular domains in complex with MSP beta-chain

4QT8 の概要

• エントリーDOI: 10.2210/pdb4qt8/pdb
• 関連するPDBエントリー: 2ASU 4FWW
• 分子名称: Macrophage-stimulating protein receptor, Hepatocyte growth factor-like protein, beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose, ... (4 entities in total)
• 機能のキーワード: growth factor receptor/growth factor, receptor-ligand complex, ron receptor tyrosine kinase, macrophage stimulating protein, hydrolase-signaling protein complex, hydrolase/signaling protein
• 由来する生物種: Homo sapiens (human); 詳細
• タンパク質・核酸の鎖数: 4
• 化学式量合計: 201659.30

構造登録者

Herzberg, O.,Chao, K.L. (登録日: 2014-07-07, 公開日: 2014-09-17, 最終更新日: 2024-11-20)

主引用文献

Chao, K.L.,Gorlatova, N.V.,Eisenstein, E.,Herzberg, O.
Structural basis for the binding specificity of human Recepteur d'Origine Nantais (RON) receptor tyrosine kinase to macrophage-stimulating protein.
J.Biol.Chem., 289:29948-29960, 2014

PubMed Abstract: Recepteur d'origine nantais (RON) receptor tyrosine kinase and its ligand, serum macrophage-stimulating protein (MSP), play important roles in inflammation, cell growth, migration, and epithelial to mesenchymal transition during tumor development. The binding of mature MSPαβ (disulfide-linked α- and β-chains) to RON ectodomain modulates receptor dimerization, followed by autophosphorylation of tyrosines in the cytoplasmic receptor kinase domains. Receptor recognition is mediated by binding of MSP β-chain (MSPβ) to the RON Sema. Here we report the structure of RON Sema-PSI-IPT1 (SPI1) domains in complex with MSPβ at 3.0 Å resolution. The MSPβ serine protease-like β-barrel uses the degenerate serine protease active site to recognize blades 2, 3, and 4 of the β-propeller fold of RON Sema. Despite the sequence homology between RON and MET receptor tyrosine kinase and between MSP and hepatocyte growth factor, it is well established that there is no cross-reactivity between the two receptor-ligand systems. Comparison of the structure of RON SPI1 in complex with MSPβ and that of MET receptor tyrosine kinase Sema-PSI in complex with hepatocyte growth factor β-chain reveals the receptor-ligand selectivity determinants. Analytical ultracentrifugation studies of the SPI1-MSPβ interaction confirm the formation of a 1:1 complex. SPI1 and MSPαβ also associate primarily as a 1:1 complex with a binding affinity similar to that of SPI1-MSPβ. In addition, the SPI1-MSPαβ ultracentrifuge studies reveal a low abundance 2:2 complex with ∼ 10-fold lower binding affinity compared with the 1:1 species. These results support the hypothesis that the α-chain of MSPαβ mediates RON dimerization.

PubMed: 25193665
DOI: 10.1074/jbc.M114.594341

実験手法

X-RAY DIFFRACTION (3 Å)