4QQN

Protein arginine methyltransferase 3 in complex with compound MTV044246

Summary for 4QQN

• Entry DOI: 10.2210/pdb4qqn/pdb
• Descriptor: PRMT3 protein, 1-{2-[1-(aminomethyl)cyclohexyl]ethyl}-3-isoquinolin-6-ylurea, GLYCEROL, ... (6 entities in total)
• Functional Keywords: prmt3, structural genomics, structural genomics consortium, sgc, transferase, epigenetics
• Biological source: Homo sapiens (human)
• Total number of polymer chains: 1
• Total formula weight: 38735.90

Authors

Dong, A.,Dobrovetsky, E.,Tempel, W.,He, H.,Zhao, K.,Smil, D.,Landon, M.,Luo, X.,Chen, Z.,Dai, M.,Yu, Z.,Lin, Y.,Zhang, H.,Zhao, K.,Schapira, M.,Brown, P.J.,Bountra, C.,Arrowsmith, C.H.,Edwards, A.M.,Vedadi, M.,Structural Genomics Consortium (SGC) (deposition date: 2014-06-27, release date: 2014-09-17, Last modification date: 2023-09-20)

Primary citation

Kaniskan, H.U.,Eram, M.S.,Zhao, K.,Szewczyk, M.M.,Yang, X.,Schmidt, K.,Luo, X.,Xiao, S.,Dai, M.,He, F.,Zang, I.,Lin, Y.,Li, F.,Dobrovetsky, E.,Smil, D.,Min, S.J.,Lin-Jones, J.,Schapira, M.,Atadja, P.,Li, E.,Barsyte-Lovejoy, D.,Arrowsmith, C.H.,Brown, P.J.,Liu, F.,Yu, Z.,Vedadi, M.,Jin, J.
Discovery of Potent and Selective Allosteric Inhibitors of Protein Arginine Methyltransferase 3 (PRMT3).
J. Med. Chem., 61:1204-1217, 2018

PubMed Abstract: PRMT3 catalyzes the asymmetric dimethylation of arginine residues of various proteins. It is crucial for maturation of ribosomes and has been implicated in several diseases. We recently disclosed a highly potent, selective, and cell-active allosteric inhibitor of PRMT3, compound 4. Here, we report comprehensive structure-activity relationship studies that target the allosteric binding site of PRMT3. We conducted design, synthesis, and evaluation of novel compounds in biochemical, selectivity, and cellular assays that culminated in the discovery of 4 and other highly potent (IC values: ∼10-36 nM), selective, and cell-active allosteric inhibitors of PRMT3 (compounds 29, 30, 36, and 37). In addition, we generated compounds that are very close analogs of these potent inhibitors but displayed drastically reduced potency as negative controls (compounds 49-51). These inhibitors and negative controls are valuable chemical tools for the biomedical community to further investigate biological functions and disease associations of PRMT3.

PubMed: 29244490
DOI: 10.1021/acs.jmedchem.7b01674

Experimental method

X-RAY DIFFRACTION (2.08 Å)