4QFG

Structure of AMPK in complex with STAUROSPORINE inhibitor and in the absence of a synthetic activator

Summary for 4QFG

• Entry DOI: 10.2210/pdb4qfg/pdb
• Related: 4QFS 4QFT
• Descriptor: 5'-AMP-activated protein kinase catalytic subunit alpha-1, 5'-AMP-activated protein kinase subunit beta-1, 5'-AMP-activated protein kinase subunit gamma-1, ... (7 entities in total)
• Functional Keywords: cbm, kinase, ampk, signaling protein-inhibitor complex, signaling protein/inhibitor
• Biological source: Rattus norvegicus (brown rat,rat,rats); More
• Cellular location: Cytoplasm : P54645
• Total number of polymer chains: 3
• Total formula weight: 119466.80

Authors

Calabrese, M.F.,Kurumbail, R.G. (deposition date: 2014-05-20, release date: 2014-08-06, Last modification date: 2024-11-06)

Primary citation

Calabrese, M.F.,Rajamohan, F.,Harris, M.S.,Caspers, N.L.,Magyar, R.,Withka, J.M.,Wang, H.,Borzilleri, K.A.,Sahasrabudhe, P.V.,Hoth, L.R.,Geoghegan, K.F.,Han, S.,Brown, J.,Subashi, T.A.,Reyes, A.R.,Frisbie, R.K.,Ward, J.,Miller, R.A.,Landro, J.A.,Londregan, A.T.,Carpino, P.A.,Cabral, S.,Smith, A.C.,Conn, E.L.,Cameron, K.O.,Qiu, X.,Kurumbail, R.G.
Structural Basis for AMPK Activation: Natural and Synthetic Ligands Regulate Kinase Activity from Opposite Poles by Different Molecular Mechanisms.
Structure, 22:1161-1172, 2014

PubMed Abstract: AMP-activated protein kinase (AMPK) is a principal metabolic regulator affecting growth and response to cellular stress. Comprised of catalytic and regulatory subunits, each present in multiple forms, AMPK is best described as a family of related enzymes. In recent years, AMPK has emerged as a desirable target for modulation of numerous diseases, yet clinical therapies remain elusive. Challenges result, in part, from an incomplete understanding of the structure and function of full-length heterotrimeric complexes. In this work, we provide the full-length structure of the widely expressed α1β1γ1 isoform of mammalian AMPK, along with detailed kinetic and biophysical characterization. We characterize binding of the broadly studied synthetic activator A769662 and its analogs. Our studies follow on the heels of the recent disclosure of the α2β1γ1 structure and provide insight into the distinct molecular mechanisms of AMPK regulation by AMP and A769662.

PubMed: 25066137
DOI: 10.1016/j.str.2014.06.009

Experimental method

X-RAY DIFFRACTION (3.46 Å)