4QFG

Structure of AMPK in complex with STAUROSPORINE inhibitor and in the absence of a synthetic activator

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	5'-AMP-activated protein kinase catalytic subunit alpha-1	polymer	503	57779.1	1	UniProt (P54645) Pfam (PF00069) Pfam (PF21147) Pfam (PF16579)	Rattus norvegicus (brown rat rat rats)	AMPK subunit alpha-1, Acetyl-CoA carboxylase kinase, ACACA kinase, Hydroxymethylglutaryl-CoA reductase kinase, HMGCR kinase, Tau-protein kinase PRKAA1
2	B (B)	5'-AMP-activated protein kinase subunit beta-1	polymer	204	23045.3	1	UniProt (P80386) Pfam (PF16561) Pfam (PF04739)	Rattus norvegicus (brown rat rat rats)	AMPK subunit beta-1, AMPKb, 5'-AMP-activated protein kinase 40 kDa subunit
3	C (C)	5'-AMP-activated protein kinase subunit gamma-1	polymer	330	37434.1	1	UniProt (P80385) Pfam (PF00571)	Rattus norvegicus (brown rat rat rats)	AMPK gamma1, AMPK subunit gamma-1, AMPKg
4	D (A)	STAUROSPORINE	non-polymer		466.5	1	Chemie (STU)
5	E, F, G (A)	CHLORIDE ION	non-polymer		35.5	3	Chemie (CL)
6	H, J, K (A, C)	SULFATE ION	non-polymer		96.1	3	Chemie (SO4)
7	I (C)	ADENOSINE MONOPHOSPHATE	non-polymer		347.2	1	Chemie (AMP)

Sequence modifications

A: 0 - 469 (UniProt: P54645)

PDB	External Database	Details
Gly -1	-	EXPRESSION TAG

A: 525 - 548 (UniProt: P54645)

PDB	External Database	Details
Ala 517	-	SEE REMARK 999
Ser 518	-	SEE REMARK 999
Gly 519	-	SEE REMARK 999
Gly 520	-	SEE REMARK 999
Pro 521	-	SEE REMARK 999
Gly 522	-	SEE REMARK 999
Gly 523	-	SEE REMARK 999
Ser 524	-	SEE REMARK 999

B: 68 - 270 (UniProt: P80386)

PDB	External Database	Details
Met 67	-	EXPRESSION TAG
Asp 108	Ser 107	ENGINEERED MUTATION

Contents of the asymmetric unit

Polymers	Number of chains	3
	Total formula weight	118258.5
Non-Polymers*	Number of molecules	8
	Total formula weight	1208.3
All*	Total formula weight	119466.8

*Water molecules are not included.