4QEP

crystal structure of KRYPTONITE in complex with mCHG DNA and SAH

Summary for 4QEP

• Entry DOI: 10.2210/pdb4qep/pdb
• Related: 4QEN 4QEO
• Descriptor: Histone-lysine N-methyltransferase, H3 lysine-9 specific SUVH4, DNA (5'-D(*GP*GP*TP*AP*CP*TP*(5CM)P*AP*GP*CP*AP*GP*TP*AP*T)-3'), DNA (5'-D(*AP*CP*TP*GP*CP*TP*GP*AP*GP*TP*AP*CP*CP*AP*T)-3'), ... (5 entities in total)
• Functional Keywords: sra, set, histone methylation, methylated dna, methylation, transcription-dna complex, transcription/dna
• Biological source: Arabidopsis thaliana (mouse-ear cress,thale-cress)
• Cellular location: Nucleus: Q8GZB6
• Total number of polymer chains: 3
• Total formula weight: 69524.87

Authors

Du, J.,Li, S.,Patel, D.J. (deposition date: 2014-05-17, release date: 2014-07-30, Last modification date: 2023-09-20)

Primary citation

Du, J.,Johnson, L.M.,Groth, M.,Feng, S.,Hale, C.J.,Li, S.,Vashisht, A.A.,Gallego-Bartolome, J.,Wohlschlegel, J.A.,Patel, D.J.,Jacobsen, S.E.
Mechanism of DNA Methylation-Directed Histone Methylation by KRYPTONITE.
Mol.Cell, 55:495-504, 2014

PubMed Abstract: In Arabidopsis, CHG DNA methylation is controlled by the H3K9 methylation mark through a self-reinforcing loop between DNA methyltransferase CHROMOMETHYLASE3 (CMT3) and H3K9 histone methyltransferase KRYPTONITE/SUVH4 (KYP). We report on the structure of KYP in complex with methylated DNA, substrate H3 peptide, and cofactor SAH, thereby defining the spatial positioning of the SRA domain relative to the SET domain. The methylated DNA is bound by the SRA domain with the 5mC flipped out of the DNA, while the H3(1-15) peptide substrate binds between the SET and post-SET domains, with the ε-ammonium of K9 positioned adjacent to bound SAH. These structural insights, complemented by functional data on key mutants of residues lining the 5mC and H3K9-binding pockets within KYP, establish how methylated DNA recruits KYP to the histone substrate. Together, the structures of KYP and previously reported CMT3 complexes provide insights into molecular mechanisms linking DNA and histone methylation.

PubMed: 25018018
DOI: 10.1016/j.molcel.2014.06.009

Experimental method

X-RAY DIFFRACTION (3.1 Å)