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4QBZ

Crystal structure of human TLR8 in complex with DS-802

Summary for 4QBZ
Entry DOI10.2210/pdb4qbz/pdb
Related4QC0
DescriptorToll-like receptor 8, alpha-D-mannopyranose-(2-3)-[beta-D-mannopyranose-(1-6)]beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose, 2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose, ... (8 entities in total)
Functional Keywordsleucine rich repeat, glycosylation, innate immunity, rna recognition, ssrna, rna binding, secreted, structure-based ligand design, tlr8 and tlr7 agonist, vaccine adjuvants, rna receptor, antiviral and antitumor drug binding, rna binding protein
Biological sourceHomo sapiens (human)
Total number of polymer chains2
Total formula weight192267.99
Authors
Tanji, H.,Ohto, U.,Shimizu, T. (deposition date: 2014-05-09, release date: 2014-10-22, Last modification date: 2024-10-09)
Primary citationYoo, E.,Salunke, D.B.,Sil, D.,Guo, X.,Salyer, A.C.D.,Hermanson, A.R.,Kumar, M.,Malladi, S.S.,Balakrishna, R.,Thompson, W.H.,Tanji, H.,Ohto, U.,Shimizu, T.,David, S.A.
Determinants of Activity at Human Toll-like Receptors 7 and 8: Quantitative Structure-Activity Relationship (QSAR) of Diverse Heterocyclic Scaffolds
J.Med.Chem., 57:7955-7970, 2014
Cited by
PubMed Abstract: Toll-like receptor (TLR) 7 and 8 agonists are potential vaccine adjuvants, since they directly activate APCs and enhance Th1-driven immune responses. Previous SAR investigations in several scaffolds of small molecule TLR7/8 activators pointed to the strict dependence of the selectivity for TLR7 vis-à-vis TLR8 on the electronic configurations of the heterocyclic systems, which we sought to examine quantitatively with the goal of developing "heuristics" to define structural requisites governing activity at TLR7 and/or TLR8. We undertook a scaffold-hopping approach, entailing the syntheses and biological evaluations of 13 different chemotypes. Crystal structures of TLR8 in complex with the two most active compounds confirmed important binding interactions playing a key role in ligand occupancy and biological activity. Density functional theory based quantum chemical calculations on these compounds followed by linear discriminant analyses permitted the classification of inactive, TLR8-active, and TLR7/8 dual-active compounds, confirming the critical role of partial charges in determining biological activity.
PubMed: 25192394
DOI: 10.1021/jm500744f
PDB entries with the same primary citation
Experimental method
X-RAY DIFFRACTION (2 Å)
Structure validation

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