4Q3M

Crystal structure of MGS-M4, an aldo-keto reductase enzyme from a Medee basin deep-sea metagenome library

Summary for 4Q3M

• Entry DOI: 10.2210/pdb4q3m/pdb
• Related: 4Q3K 4Q3L 4Q3N 4Q3O
• Descriptor: MGS-M4, SULFATE ION, SODIUM ION, ... (4 entities in total)
• Functional Keywords: metagenome, metagenomic library, oxidoreductase, alpha/beta barrel, tim barrel, hydrolase, unknown substrate
• Biological source: unidentified
• Total number of polymer chains: 6
• Total formula weight: 191604.95

Authors

Stogios, P.J.,Xu, X.,Cui, H.,Alcaide, M.,Ferrer, M.,Savchenko, A. (deposition date: 2014-04-11, release date: 2015-02-25, Last modification date: 2023-09-20)

Primary citation

Alcaide, M.,Stogios, P.J.,Lafraya, A.,Tchigvintsev, A.,Flick, R.,Bargiela, R.,Chernikova, T.N.,Reva, O.N.,Hai, T.,Leggewie, C.C.,Katzke, N.,La Cono, V.,Matesanz, R.,Jebbar, M.,Jaeger, K.E.,Yakimov, M.M.,Yakunin, A.F.,Golyshin, P.N.,Golyshina, O.V.,Savchenko, A.,Ferrer, M.
Pressure adaptation is linked to thermal adaptation in salt-saturated marine habitats.
Environ Microbiol, 17:332-345, 2015

PubMed Abstract: The present study provides a deeper view of protein functionality as a function of temperature, salt and pressure in deep-sea habitats. A set of eight different enzymes from five distinct deep-sea (3040-4908 m depth), moderately warm (14.0-16.5°C) biotopes, characterized by a wide range of salinities (39-348 practical salinity units), were investigated for this purpose. An enzyme from a 'superficial' marine hydrothermal habitat (65°C) was isolated and characterized for comparative purposes. We report here the first experimental evidence suggesting that in salt-saturated deep-sea habitats, the adaptation to high pressure is linked to high thermal resistance (P value = 0.0036). Salinity might therefore increase the temperature window for enzyme activity, and possibly microbial growth, in deep-sea habitats. As an example, Lake Medee, the largest hypersaline deep-sea anoxic lake of the Eastern Mediterranean Sea, where the water temperature is never higher than 16°C, was shown to contain halopiezophilic-like enzymes that are most active at 70°C and with denaturing temperatures of 71.4°C. The determination of the crystal structures of five proteins revealed unknown molecular mechanisms involved in protein adaptation to poly-extremes as well as distinct active site architectures and substrate preferences relative to other structurally characterized enzymes.

PubMed: 25330254
DOI: 10.1111/1462-2920.12660

Experimental method

X-RAY DIFFRACTION (2.552 Å)