4PZ3
High-resolution crystal structure of the human CD44 hyaluronan binding domain complex with undefined peptides
Summary for 4PZ3
| Entry DOI | 10.2210/pdb4pz3/pdb |
| Related | 1UUH 4PZ4 |
| Descriptor | CD44 antigen, Undefined peptides modeled as AAAV, DI(HYDROXYETHYL)ETHER, ... (6 entities in total) |
| Functional Keywords | link module, binding protein, hyaluronan, ectodomain, cell adhesion |
| Biological source | Homo sapiens (human) More |
| Cellular location | Cell membrane; Single-pass type I membrane protein: P16070 |
| Total number of polymer chains | 3 |
| Total formula weight | 34675.80 |
| Authors | Liu, L.K.,Finzel, B. (deposition date: 2014-03-28, release date: 2014-09-17, Last modification date: 2024-10-16) |
| Primary citation | Liu, L.K.,Finzel, B. High-resolution crystal structures of alternate forms of the human CD44 hyaluronan-binding domain reveal a site for protein interaction. Acta Crystallogr F Struct Biol Commun, 70:1155-1161, 2014 Cited by PubMed Abstract: Two new crystal structures of the extracellular hyaluronan-binding domain of human CD44 are described at high resolution. A hexagonal crystal form at 1.60 Å resolution and a monoclinic form at 1.08 Å resolution both have two molecules in the asymmetric unit arranged about a similar noncrystallographic twofold axis of symmetry. These structures are compared with those previously reported at 2.20 Å resolution to show that the fold is quite resistant to structural deformation in different crystal environments. Unexpectedly, a short peptide is found in the monoclinic crystals at a site remote from the known hyaluronan-binding groove. The peptide with a valine at the carboxy-terminus must have co-purified from the bacterial expression host and binds on the opposite side of the domain from the known hyaluronan-binding groove. This opportunistic binding may identify a site of interaction used as CD44 assembles with other proteins to accomplish effective signaling regarding changes to the extracellular environment. PubMed: 25195884DOI: 10.1107/S2053230X14015532 PDB entries with the same primary citation |
| Experimental method | X-RAY DIFFRACTION (1.083 Å) |
Structure validation
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