4PU3

Shewanella oneidensis MR-1 Toxin Antitoxin System HipA, HipB and its operator DNA complex (space group P212121)

Summary for 4PU3

• Entry DOI: 10.2210/pdb4pu3/pdb
• Related: 4PU4 4PU5
• Descriptor: Toxin-antitoxin system toxin HipA family, Toxin-antitoxin system antidote transcriptional repressor Xre family, Operator DNA, ... (4 entities in total)
• Functional Keywords: toxin antitoxin system, toxin-antitoxin-dna complex, toxin/antitoxin/dna
• Biological source: Shewanella oneidensis; More
• Total number of polymer chains: 6
• Total formula weight: 144207.75

Authors

Wen, Y.,Behiels, E.,Felix, J.,Elegheert, J.,Vergauwen, B.,Devreese, B.,Savvides, S. (deposition date: 2014-03-12, release date: 2014-08-06, Last modification date: 2024-11-06)

Primary citation

Wen, Y.,Behiels, E.,Felix, J.,Elegheert, J.,Vergauwen, B.,Devreese, B.,Savvides, S.N.
The bacterial antitoxin HipB establishes a ternary complex with operator DNA and phosphorylated toxin HipA to regulate bacterial persistence.
Nucleic Acids Res., 42:10134-10147, 2014

PubMed Abstract: Nearly all bacteria exhibit a type of phenotypic growth described as persistence that is thought to underlie antibiotic tolerance and recalcitrant chronic infections. The chromosomally encoded high-persistence (Hip) toxin-antitoxin proteins HipASO and HipBSO from Shewanella oneidensis, a proteobacterium with unusual respiratory capacities, constitute a type II toxin-antitoxin protein module. Here we show that phosphorylated HipASO can engage in an unexpected ternary complex with HipBSO and double-stranded operator DNA that is distinct from the prototypical counterpart complex from Escherichia coli. The structure of HipBSO in complex with operator DNA reveals a flexible C-terminus that is sequestered by HipASO in the ternary complex, indicative of its role in binding HipASO to abolish its function in persistence. The structure of HipASO in complex with a non-hydrolyzable ATP analogue shows that HipASO autophosphorylation is coupled to an unusual conformational change of its phosphorylation loop. However, HipASO is unable to phosphorylate the translation factor Elongation factor Tu, contrary to previous reports, but in agreement with more recent findings. Our studies suggest that the phosphorylation state of HipA is an important factor in persistence and that the structural and mechanistic diversity of HipAB modules as regulatory factors in bacterial persistence is broader than previously thought.

PubMed: 25056321
DOI: 10.1093/nar/gku665

Experimental method

X-RAY DIFFRACTION (3.39 Å)