4PSX

Crystal structure of histone acetyltransferase complex

Summary for 4PSX

• Entry DOI: 10.2210/pdb4psx/pdb
• Descriptor: Histone acetyltransferase type B catalytic subunit, Histone acetyltransferase type B subunit 2, Histone H4, ... (7 entities in total)
• Functional Keywords: hat wd40, acetyltransferase, accoa, phosphorylation, histone-transferase complex, histone/transferase
• Biological source: Saccharomyces cerevisiae (Baker's yeast); More
• Cellular location: Cytoplasm: Q12341 P39984; Nucleus: P02309 P61830
• Total number of polymer chains: 8
• Total formula weight: 180297.38

Authors

Yang, M.,Li, Y. (deposition date: 2014-03-08, release date: 2014-07-09, Last modification date: 2023-11-08)

Primary citation

Li, Y.,Zhang, L.,Liu, T.,Chai, C.,Fang, Q.,Wu, H.,Agudelo garcia, P.A.,Han, Z.,Zong, S.,Yu, Y.,Zhang, X.,Parthun, M.R.,Chai, J.,Xu, R.M.,Yang, M.
Hat2p recognizes the histone H3 tail to specify the acetylation of the newly synthesized H3/H4 heterodimer by the Hat1p/Hat2p complex
Genes Dev., 28:1217-1227, 2014

PubMed Abstract: Post-translational modifications of histones are significant regulators of replication, transcription, and DNA repair. Particularly, newly synthesized histone H4 in H3/H4 heterodimers becomes acetylated on N-terminal lysine residues prior to its incorporation into chromatin. Previous studies have established that the histone acetyltransferase (HAT) complex Hat1p/Hat2p medicates this modification. However, the mechanism of how Hat1p/Hat2p recognizes and facilitates the enzymatic activities on the newly assembled H3/H4 heterodimer remains unknown. Furthermore, Hat2p is a WD40 repeat protein, which is found in many histone modifier complexes. However, how the WD40 repeat proteins facilitate enzymatic activities of histone modification enzymes is unclear. In this study, we first solved the high-resolution crystal structure of a Hat1p/Hat2p/CoA/H4 peptide complex and found that the H4 tail interacts with both Hat1p and Hat2p, by which substrate recruitment is facilitated. We further discovered that H3 N-terminal peptides can bind to the Hat2p WD40 domain and solved the structure of the Hat1p/Hat2p/CoA/H4/H3 peptide complex. Moreover, the interaction with Hat2p requires unmodified Arg2/Lys4 and Lys9 on the H3 tail, suggesting a novel model to specify the activity of Hat1p/Hat2p toward newly synthesized H3/H4 heterodimers. Together, our study demonstrated the substrate recognition mechanism by the Hat1p/Hat2p complex, which is critical for DNA replication and other chromatin remodeling processes.

PubMed: 24835250
DOI: 10.1101/gad.240531.114

Experimental method

X-RAY DIFFRACTION (2.509 Å)