4PPX

DNA Polymerase Beta E295K with Spiroiminodihydantoin in Templating Position

Summary for 4PPX

• Entry DOI: 10.2210/pdb4ppx/pdb
• Descriptor: DNA polymerase beta, 5'-D(*CP*CP*GP*AP*CP*(SDH)P*GP*CP*GP*CP*AP*TP*CP*AP*GP*C)-3', 5'-D(*GP*CP*TP*GP*AP*TP*GP*CP*GP*C)-3', ... (6 entities in total)
• Functional Keywords: dna polymerase, dna complex, transferase, lyase-dna complex, lyase/dna
• Biological source: Homo sapiens (human)
• Cellular location: Nucleus: P06746
• Total number of polymer chains: 4
• Total formula weight: 47787.93

Authors

Eckenroth, B.E.,Doublie, S. (deposition date: 2014-02-27, release date: 2014-04-02, Last modification date: 2023-09-20)

Primary citation

Eckenroth, B.E.,Fleming, A.M.,Sweasy, J.B.,Burrows, C.J.,Doublie, S.
Crystal Structure of DNA Polymerase beta with DNA Containing the Base Lesion Spiroiminodihydantoin in a Templating Position.
Biochemistry, 53:2075-2077, 2014

PubMed Abstract: The first high-resolution crystal structure of spiroiminodihydantoin (dSp1) was obtained in the context of the DNA polymerase β active site and reveals two areas of significance. First, the structure verifies the recently determined S configuration at the spirocyclic carbon. Second, the distortion of the DNA duplex is similar to that of the single-oxidation product 8-oxoguanine. For both oxidized lesions, adaptation of the syn conformation results in similar backbone distortions in the DNA duplex. The resulting conformation positions the dSp1 A-ring as the base-pairing face whereas the B-ring of dSp1 protrudes into the major groove.

PubMed: 24649945
DOI: 10.1021/bi500270e

Experimental method

X-RAY DIFFRACTION (2.08 Å)