4POY

Engineered Dual Specific VHH antibody

Summary for 4POY

• Entry DOI: 10.2210/pdb4poy/pdb
• Related: 4POU
• Descriptor: VHH-metal (2 entities in total)
• Functional Keywords: single domain antibody, vhh, metalloregulation, bispecific, dual-function, protein engineering, affinity control, protein-switch, antibody engineering, nickel binding protein, protein-metal coordination geometry, loop dynamics, protein flexibility, conformational dynamics, binding thermodynamics, antibody-metal complex, immune system
• Biological source: Lama glama
• Total number of polymer chains: 1
• Total formula weight: 12941.36

Authors

Fanning, S.W.,Walter, R.,Horn, J.R. (deposition date: 2014-02-26, release date: 2014-09-24, Last modification date: 2014-10-22)

• Primary citation: Fanning, S.W.,Walter, R.,Horn, J.R.; Structural basis of an engineered dual-specific antibody: conformational diversity leads to a hypervariable loop metal-binding site.; Protein Eng.Des.Sel., 27:391-397, 2014; PubMed: 25143596; DOI: 10.1093/protein/gzu033

Experimental method

X-RAY DIFFRACTION (1.5 Å)