4POY
Engineered Dual Specific VHH antibody
Experimental procedure
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 22-BM |
Synchrotron site | APS |
Beamline | 22-BM |
Detector technology | CCD |
Collection date | 2010-06-15 |
Detector | MAR CCD 165 mm |
Wavelength(s) | 1.00 |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 53.753, 61.979, 64.803 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 19.380 - 1.500 |
R-factor | 0.20259 |
Rwork | 0.201 |
R-free | 0.24123 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.016 |
RMSD bond angle | 1.516 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | REFMAC (5.5.0102) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 50.000 |
High resolution limit [Å] | 1.300 |
Rmerge | 0.038 |
Number of reflections | 191128 |
Completeness [%] | 99.8 |
Redundancy | 7.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 298.15 | 0.1 M Bis-Tris, 2.0 M Ammonium Sulfate, pH 5.5, VAPOR DIFFUSION, HANGING DROP, temperature 298.15K |