4P69

Acek (D477A) ICDH complex

Summary for 4P69

Entry DOI	10.2210/pdb4p69/pdb
Descriptor	Isocitrate dehydrogenase kinase/phosphatase, Isocitrate dehydrogenase [NADP], ADENOSINE MONOPHOSPHATE, ... (5 entities in total)
Functional Keywords	transferase, hydrolase-oxidoreductase complex, hydrolase/oxidoreductase
Biological source	Escherichia coli O157:H7 More
Cellular location	Cytoplasm : Q8X607
Total number of polymer chains	4
Total formula weight	225897.55
Authors	Jimin, Z.,Nan, W.,Shu, W.,Zongchao, J. (deposition date: 2014-03-22, release date: 2014-10-08, Last modification date: 2023-12-27)
Primary citation	Wang, S.,Shen, Q.,Chen, G.,Zheng, J.,Tan, H.,Jia, Z. The phosphatase mechanism of bifunctional kinase/phosphatase AceK. Chem. Commun. (Camb.), 50:14117-14120, 2014 Cited by PubMed Abstract: We have revealed that bifunctional AceK kinase/phosphatase utilizes a stepwise addition-elimination mechanism in its dephosphorylation reaction. This work explains how AceK enables opposite kinase and phosphatase activities with Asp477 and a single Mg(2+) ion. PubMed: 25272278 DOI: 10.1039/c4cc05375c PDB entries with the same primary citation
Experimental method	X-RAY DIFFRACTION (3.3 Å)

Structure validation