4P5H

Structure of Clostridium perfringens Enterotoxin with a peptide derived from a modified version of ECL-2 of Claudin 2

Replaces:  3ZJ3

Summary for 4P5H

• Entry DOI: 10.2210/pdb4p5h/pdb
• Descriptor: Claudin-2, Heat-labile enterotoxin B chain (2 entities in total)
• Functional Keywords: toxin-cell adhesion complex, beta pore-forming toxin, receptor binding, toxin/cell adhesion
• Biological source: Clostridium perfringens; More
• Total number of polymer chains: 30
• Total formula weight: 508540.96

Authors

Naylor, C.E.,Yelland, T.S.,Basak, A.K. (deposition date: 2014-03-17, release date: 2014-07-16, Last modification date: 2023-09-27)

Primary citation

Yelland, T.S.,Naylor, C.E.,Bagoban, T.,Savva, C.G.,Moss, D.S.,McClane, B.A.,Blasig, I.E.,Popoff, M.,Basak, A.K.
Structure of a C. perfringens Enterotoxin Mutant in Complex with a Modified Claudin-2 Extracellular Loop 2.
J.Mol.Biol., 426:3134-3147, 2014

PubMed Abstract: CPE (Clostridium perfringens enterotoxin) is the major virulence determinant for C. perfringens type-A food poisoning, the second most common bacterial food-borne illness in the UK and USA. After binding to its receptors, which include particular human claudins, the toxin forms pores in the cell membrane. The mature pore apparently contains a hexamer of CPE, claudin and, possibly, occludin. The combination of high binding specificity with cytotoxicity has resulted in CPE being investigated, with some success, as a targeted cytotoxic agent for oncotherapy. In this paper, we present the X-ray crystallographic structure of CPE in complex with a peptide derived from extracellular loop 2 of a modified, CPE-binding Claudin-2, together with high-resolution native and pore-formation mutant structures. Our structure provides the first atomic-resolution data on any part of a claudin molecule and reveals that claudin's CPE-binding fingerprint (NPLVP) is in a tight turn conformation and binds, as expected, in CPE's C-terminal claudin-binding groove. The leucine and valine residues insert into the binding groove while the first residue, asparagine, tethers the peptide via an interaction with CPE's aspartate 225 and the two prolines are required to maintain the tight turn conformation. Understanding the structural basis of the contribution these residues make to binding will aid in engineering CPE to target tumor cells.

PubMed: 25020226
DOI: 10.1016/j.jmb.2014.07.001

Experimental method

X-RAY DIFFRACTION (3.38 Å)