4OW1

Crystal Structure of Resuscitation Promoting Factor C

Summary for 4OW1

• Entry DOI: 10.2210/pdb4ow1/pdb
• Descriptor: Resuscitation-promoting factor RpfC, 1,2-ETHANEDIOL (3 entities in total)
• Functional Keywords: resuscitation promoting factor, peptidoglycan, transglycosylase, hydrolase
• Biological source: Mycobacterium tuberculosis
• Total number of polymer chains: 8
• Total formula weight: 74630.50

Authors

Chauviac, F.X.,Quay, D.H.X.,Cohen-Gonsaud, M.,Keep, N.H. (deposition date: 2014-01-29, release date: 2014-06-18, Last modification date: 2024-10-09)

Primary citation

Chauviac, F.X.,Robertson, G.,Quay, D.H.,Bagneris, C.,Dumas, C.,Henderson, B.,Ward, J.,Keep, N.H.,Cohen-Gonsaud, M.
The RpfC (Rv1884) atomic structure shows high structural conservation within the resuscitation-promoting factor catalytic domain.
Acta Crystallogr.,Sect.F, 70:1022-1026, 2014

PubMed Abstract: The first structure of the catalytic domain of RpfC (Rv1884), one of the resuscitation-promoting factors (RPFs) from Mycobacterium tuberculosis, is reported. The structure was solved using molecular replacement once the space group had been correctly identified as twinned P21 rather than the apparent C2221 by searching for anomalous scattering sites in P1. The structure displays a very high degree of structural conservation with the previously published structures of the catalytic domains of RpfB (Rv1009) and RpfE (Rv2450). This structural conservation highlights the importance of the versatile domain composition of the RPF family.

PubMed: 25084374
DOI: 10.1107/S2053230X1401317X

Experimental method

X-RAY DIFFRACTION (1.9 Å)