4OW1
Crystal Structure of Resuscitation Promoting Factor C
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID23-1 |
Synchrotron site | ESRF |
Beamline | ID23-1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 1999-10-06 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 1.0723 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 66.230, 89.930, 78.090 |
Unit cell angles | 90.00, 115.08, 90.00 |
Refinement procedure
Resolution | 38.420 - 1.900 |
R-factor | 0.20653 |
Rwork | 0.205 |
R-free | 0.23548 |
RMSD bond length | 0.022 |
RMSD bond angle | 1.823 |
Refinement software | REFMAC (5.8.0049) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 44.970 | 1.940 |
High resolution limit [Å] | 1.900 | 1.900 |
Rmerge | 0.076 | 0.382 |
Number of reflections | 64089 | |
<I/σ(I)> | 8.3 | 2.6 |
Completeness [%] | 99.6 | 100 |
Redundancy | 4.1 | 4.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 5 | 289 | 0.1 M NaCitrate pH 5, 22% (w/v) PEG 6000 |