4OV0

Structure of Bacteriorhdopsin Transferred from Amphipol A8-35 to a Lipidic Mesophase

Summary for 4OV0

• Entry DOI: 10.2210/pdb4ov0/pdb
• Descriptor: Bacteriorhodopsin, RETINAL (3 entities in total)
• Functional Keywords: light-driven proton pump, retinal attached via schiff base, membrane, transport protein
• Biological source: Halobacterium sp.
• Cellular location: Cell membrane ; Multi-pass membrane protein : P02945
• Total number of polymer chains: 1
• Total formula weight: 27213.94

Authors

Polovinkin, V.,Gushchin, I.,Sintsov, M.,Round, E.,Balandin, T.,Chervakov, P.,Schevchenko, V.,Utrobin, P.,Popov, A.,Borshchevskiy, V.,Mishin, A.,Kuklin, A.,Willbold, D.,Popot, J.L.,Gordeliy, V. (deposition date: 2014-02-19, release date: 2014-10-01, Last modification date: 2024-11-27)

Primary citation

Polovinkin, V.,Gushchin, I.,Sintsov, M.,Round, E.,Balandin, T.,Chervakov, P.,Schevchenko, V.,Utrobin, P.,Popov, A.,Borshchevskiy, V.,Mishin, A.,Kuklin, A.,Willbold, D.,Chupin, V.,Popot, J.L.,Gordeliy, V.
High-resolution structure of a membrane protein transferred from amphipol to a lipidic mesophase.
J.Membr.Biol., 247:997-1004, 2014

PubMed Abstract: Amphipols (APols) have become important tools for the stabilization, folding, and in vitro structural and functional studies of membrane proteins (MPs). Direct crystallization of MPs solubilized in APols would be of high importance for structural biology. However, despite considerable efforts, it is still not clear whether MP/APol complexes can form well-ordered crystals suitable for X-ray crystallography. In the present work, we show that an APol-trapped MP can be crystallized in meso. Bacteriorhodopsin (BR) trapped by APol A8-35 was mixed with a lipidic mesophase, and crystallization was induced by adding a precipitant. The crystals diffract beyond 2 Å. The structure of BR was solved to 2 Å and found to be indistinguishable from previous structures obtained after transfer from detergent solutions. We suggest the proposed protocol of in meso crystallization to be generally applicable to APol-trapped MPs.

PubMed: 25192977
DOI: 10.1007/s00232-014-9700-x

Experimental method

X-RAY DIFFRACTION (2 Å)