4OV0
Structure of Bacteriorhdopsin Transferred from Amphipol A8-35 to a Lipidic Mesophase
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID23-1 |
Synchrotron site | ESRF |
Beamline | ID23-1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2013-05-03 |
Detector | PSI PILATUS 6M |
Wavelength(s) | 0.972 |
Spacegroup name | P 63 |
Unit cell lengths | 60.999, 60.999, 108.174 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 52.826 - 2.000 |
R-factor | 0.1851 |
Rwork | 0.179 |
R-free | 0.20900 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.008 |
RMSD bond angle | 1.476 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | MOLREP |
Refinement software | PHENIX ((phenix.refine: 1.8_1069)) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 52.830 |
High resolution limit [Å] | 2.000 |
Rmerge | 0.121 |
Number of reflections | 15418 |
<I/σ(I)> | 9.7 |
Completeness [%] | 99.9 |
Redundancy | 6.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 5.6 | 295 | Monooleoyl, amphipol A8-35, 1-2M sodium/potassium phosphate, pH 5.6, VAPOR DIFFUSION, HANGING DROP, temperature 295K |