4OAU

Complete human RNase L in complex with biological activators.

4OAU の概要

• エントリーDOI: 10.2210/pdb4oau/pdb
• 関連するPDBエントリー: 4OAV
• 分子名称: 2-5A-dependent ribonuclease, RNA (5'-R(P*A*AP*A)-2'), ADENOSINE-5'-DIPHOSPHATE, ... (5 entities in total)
• 機能のキーワード: rnase l, rnasel, 2-5a, 2', 5'-oligoadenylate, interferon, ken, pseudokinase, kinase, inflammation, ire1, ridd, regulated rna decay, splicing cleavage, hpc1, hereditary prostate cancer 1, rnase l kinase-homology and ken domain-containing, innate immunity, interferon response, antiviral response, 2-5a (2', 5'-linked oligoadenylate)and rna, hydrolase-rna complex, hydrolase/rna
• 由来する生物種: Homo sapiens (human); 詳細
• 細胞内の位置: Cytoplasm: Q05823
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 80834.82

構造登録者

Han, Y.,Donovan, J.,Rath, S.,Whitney, G.,Chitrakar, A.,Korennykh, A. (登録日: 2014-01-06, 公開日: 2014-03-12, 最終更新日: 2024-02-28)

主引用文献

Han, Y.,Donovan, J.,Rath, S.,Whitney, G.,Chitrakar, A.,Korennykh, A.
Structure of human RNase L reveals the basis for regulated RNA decay in the IFN response.
Science, 343:1244-1248, 2014

PubMed Abstract: One of the hallmark mechanisms activated by type I interferons (IFNs) in human tissues involves cleavage of intracellular RNA by the kinase homology endoribonuclease RNase L. We report 2.8 and 2.1 angstrom crystal structures of human RNase L in complexes with synthetic and natural ligands and a fragment of an RNA substrate. RNase L forms a crossed homodimer stabilized by ankyrin (ANK) and kinase homology (KH) domains, which positions two kinase extension nuclease (KEN) domains for asymmetric RNA recognition. One KEN protomer recognizes an identity nucleotide (U), whereas the other protomer cleaves RNA between nucleotides +1 and +2. The coordinated action of the ANK, KH, and KEN domains thereby provides regulated, sequence-specific cleavage of viral and host RNA targets by RNase L.

PubMed: 24578532
DOI: 10.1126/science.1249845

実験手法

X-RAY DIFFRACTION (2.6 Å)