4O9R

Human Smoothened Receptor structure in complex with cyclopamine

Summary for 4O9R

• Entry DOI: 10.2210/pdb4o9r/pdb
• Descriptor: Smoothened homolog/Soluble cytochrome b562 chimeric protein, Cyclopamine (2 entities in total)
• Functional Keywords: serial femtosecond crystallography, human smo receptor, cyclopamine, novel protein engineering, gpcr network, psi-biology, free electron laser, structural genomics, membrane protein, xfel, lcp, room temperature structure, gpcr, membrane, signaling protein
• Biological source: Homo sapiens; More
• Cellular location: Membrane ; Multi-pass membrane protein : Q99835
• Total number of polymer chains: 1
• Total formula weight: 52806.82

Authors

Wang, C.,Weierstall, U.,James, D.,White, T.A.,Wang, D.,Liu, W.,Spence, J.C.H.,Doak, R.B.,Nelson, G.,Fromme, P.,Fromme, R.,Grotjohann, I.,Kupitz, C.,Zatsepin, N.A.,Liu, H.,Basu, S.,Wacker, D.,Han, G.W.,Katritch, V.,Boutet, S.,Messerschmidt, M.,Willams, G.J.,Koglin, J.E.,Seibert, M.M.,Klinker, M.,Gati, C.,Shoeman, R.L.,Barty, A.,Chapman, H.N.,Kirian, R.A.,Beyerlein, K.R.,Stevens, R.C.,Li, D.,Shah, S.T.A.,Howe, N.,Caffrey, M.,Cherezov, V.,GPCR Network (GPCR) (deposition date: 2014-01-02, release date: 2014-03-05, Last modification date: 2024-10-16)

Primary citation

Weierstall, U.,James, D.,Wang, C.,White, T.A.,Wang, D.,Liu, W.,Spence, J.C.,Bruce Doak, R.,Nelson, G.,Fromme, P.,Fromme, R.,Grotjohann, I.,Kupitz, C.,Zatsepin, N.A.,Liu, H.,Basu, S.,Wacker, D.,Han, G.W.,Katritch, V.,Boutet, S.,Messerschmidt, M.,Williams, G.J.,Koglin, J.E.,Marvin Seibert, M.,Klinker, M.,Gati, C.,Shoeman, R.L.,Barty, A.,Chapman, H.N.,Kirian, R.A.,Beyerlein, K.R.,Stevens, R.C.,Li, D.,Shah, S.T.,Howe, N.,Caffrey, M.,Cherezov, V.
Lipidic cubic phase injector facilitates membrane protein serial femtosecond crystallography.
Nat Commun, 5:3309-3309, 2014

PubMed Abstract: Lipidic cubic phase (LCP) crystallization has proven successful for high-resolution structure determination of challenging membrane proteins. Here we present a technique for extruding gel-like LCP with embedded membrane protein microcrystals, providing a continuously renewed source of material for serial femtosecond crystallography. Data collected from sub-10-μm-sized crystals produced with less than 0.5 mg of purified protein yield structural insights regarding cyclopamine binding to the Smoothened receptor.

PubMed: 24525480
DOI: 10.1038/ncomms4309

Experimental method

X-RAY DIFFRACTION (3.204 Å)