Human Smoothened Receptor structure in complex with cyclopamine

Summary for 4O9R

DescriptorSmoothened homolog/Soluble cytochrome b562 chimeric protein, Cyclopamine (2 entities in total)
Functional Keywordsserial femtosecond crystallography, human smo receptor, cyclopamine, novel protein engineering, gpcr network, psi-biology, free electron laser, structural genomics, membrane protein, xfel, lcp, room temperature structure, gpcr, membrane, signaling protein
Biological sourceHomo sapiens
Cellular locationMembrane ; Multi-pass membrane protein  Q99835
Total number of polymer chains1
Total molecular weight52806.82
Primary citation
Weierstall, U.,James, D.,Wang, C.,White, T.A.,Wang, D.,Liu, W.,Spence, J.C.,Bruce Doak, R.,Nelson, G.,Fromme, P.,Fromme, R.,Grotjohann, I.,Kupitz, C.,Zatsepin, N.A.,Liu, H.,Basu, S.,Wacker, D.,Han, G.W.,Katritch, V.,Boutet, S.,Messerschmidt, M.,Williams, G.J.,Koglin, J.E.,Marvin Seibert, M.,Klinker, M.,Gati, C.,Shoeman, R.L.,Barty, A.,Chapman, H.N.,Kirian, R.A.,Beyerlein, K.R.,Stevens, R.C.,Li, D.,Shah, S.T.,Howe, N.,Caffrey, M.,Cherezov, V.
Lipidic cubic phase injector facilitates membrane protein serial femtosecond crystallography.
Nat Commun, 5:3309-3309, 2014
PubMed: 24525480 (PDB entries with the same primary citation)
DOI: 10.1038/ncomms4309
MImport into Mendeley
Experimental method

Structure validation

RfreeClashscoreRamachandran outliersSidechain outliersRSRZ outliers0.283202.6%2.3%MetricValuePercentile RanksWorseBetterPercentile relative to all X-ray structuresPercentile relative to X-ray structures of similar resolution
Download full validation reportDownload