4O8U

Structure of PF2046

Summary for 4O8U

• Entry DOI: 10.2210/pdb4o8u/pdb
• Descriptor: Uncharacterized protein PF2046 (2 entities in total)
• Functional Keywords: low resolution diffraction, pf2046, b-factorsharpening, homodimer of trimers, unknown function
• Biological source: Pyrococcus furiosus
• Total number of polymer chains: 6
• Total formula weight: 154357.10

Authors

Su, J.,Liu, Z.-J. (deposition date: 2013-12-30, release date: 2014-04-30, Last modification date: 2024-11-06)

Primary citation

Su, J.,Li, Y.,Shaw, N.,Zhou, W.H.,Zhang, M.,Xu, H.,Wang, B.-C.,Liu, Z.-J.
Crystal structure of a novel non-Pfam protein PF2046 solved using low resolution B-factor sharpening and multi-crystal averaging methods
Protein Cell, 1:453-458, 2010

PubMed Abstract: Sometimes crystals cannot diffract X-rays beyond 3.0 Å resolution due to the intrinsic flexibility associated with the protein. Low resolution diffraction data not only pose a challenge to structure determination, but also hamper interpretation of mechanistic details. Crystals of a 25.6 kDa non-Pfam, hypothetical protein, PF2046, diffracted X-rays to 3.38 Å resolution. A combination of Se-Met derived heavy atom positions with multiple cycles of B-factor sharpening, multi-crystal averaging, restrained refinement followed by manual inspection of electron density and model building resulted in a final model with a R value of 23.5 (R(free)= 24.7). The asymmetric unit was large and consisted of six molecules arranged as a homodimer of trimers. Analysis of the structure revealed the presence of a RNA binding domain suggesting a role for PF2046 in the processing of nucleic acids.

PubMed: 21203960
DOI: 10.1007/s13238-010-0045-7

Experimental method

X-RAY DIFFRACTION (2.345 Å)