4O8U
Structure of PF2046
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-ID |
| Synchrotron site | APS |
| Beamline | 22-ID |
| Temperature [K] | 93 |
| Detector technology | IMAGE PLATE |
| Collection date | 2009-05-01 |
| Detector | RIGAKU RAXIS IV |
| Wavelength(s) | 0.9794 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 218.381, 125.640, 94.044 |
| Unit cell angles | 90.00, 102.86, 90.00 |
Refinement procedure
| Resolution | 36.068 - 2.345 |
| R-factor | 0.2022 |
| Rwork | 0.201 |
| R-free | 0.23780 |
| Structure solution method | SAD |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.439 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | SHARP |
| Refinement software | PHENIX ((phenix.refine: 1.8.2_1309)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.400 |
| High resolution limit [Å] | 2.345 | 2.340 |
| Number of reflections | 88068 | |
| Completeness [%] | 85.0 | 85 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5 | 289 | 20% PEG 4000, 20% (v/v) 2-propanol, 0.1M sodium citrate, 0.1M sodium acetate pH 5.0, 0.1M sodium chloride, 10% (w/v) MPD, VAPOR DIFFUSION, HANGING DROP, temperature 289K |
