4NQA

Crystal structure of liganded hRXR-alpha/hLXR-beta heterodimer on DNA

4NQA の概要

• エントリーDOI: 10.2210/pdb4nqa/pdb
• 分子名称: Retinoic acid receptor RXR-alpha, Liver X nuclear receptor beta, Nuclear receptor coactivator 2, ... (8 entities in total)
• 機能のキーワード: multi-domain, rxr, lxr, dbd, lbd, ligand, zinc finger, transcription regulator-dna complex, transcription regulator/dna
• 由来する生物種: Homo sapiens (human); 詳細
• 細胞内の位置: Nucleus : P19793 F1D8P7 Q15596
• タンパク質・核酸の鎖数: 12
• 化学式量合計: 201035.72

構造登録者

Lou, X.H.,Toresson, G.,Benod, C.,Suh, J.H.,Phillips, K.J.,Webb, P.,Gustafsson, J.A. (登録日: 2013-11-24, 公開日: 2014-02-26, 最終更新日: 2024-02-28)

主引用文献

Lou, X.,Toresson, G.,Benod, C.,Suh, J.H.,Philips, K.J.,Webb, P.,Gustafsson, J.A.
Structure of the retinoid X receptor alpha-liver X receptor beta (RXR alpha-LXR beta ) heterodimer on DNA.
Nat.Struct.Mol.Biol., 21:277-281, 2014

PubMed Abstract: Nuclear receptors (NRs) are conditional transcription factors with common multidomain organization that bind diverse DNA elements. How DNA sequences influence NR conformation is poorly understood. Here we report the crystal structure of the human retinoid X receptor α-liver X receptor β (RXRα-LXRβ) heterodimer on its cognate element, an AGGTCA direct repeat spaced by 4 nt. The complex has an extended X-shaped arrangement, with DNA- and ligand-binding domains crossed, in contrast to the parallel domain arrangement of other NRs that bind an AGGTCA direct repeat spaced by 1 nt. The LXRβ core binds DNA via canonical contacts and auxiliary DNA contacts that enhance affinity for the response element. Comparisons of RXRα-LXRβs in the crystal asymmetric unit and with previous NR structures reveal flexibility in NR organization and suggest a role for RXRα in adaptation of heterodimeric complexes to DNA.

PubMed: 24561505
DOI: 10.1038/nsmb.2778

実験手法

X-RAY DIFFRACTION (3.102 Å)