4NJ6

PB1 Domain of AtARF7

Summary for 4NJ6

• Entry DOI: 10.2210/pdb4nj6/pdb
• Related: 4NJ7
• Descriptor: Auxin response factor 7 (2 entities in total)
• Functional Keywords: pb1 domain, beta-grasp fold, protein binding
• Biological source: Arabidopsis thaliana (mouse-ear cress,thale-cress)
• Cellular location: Nucleus: P93022
• Total number of polymer chains: 16
• Total formula weight: 174195.44

Authors

Korasick, D.A.,Westfall, C.S.,Lee, S.G.,Nanao, M.,Jez, J.M.,Strader, L.C. (deposition date: 2013-11-08, release date: 2014-03-26, Last modification date: 2024-02-28)

Primary citation

Korasick, D.A.,Westfall, C.S.,Lee, S.G.,Nanao, M.H.,Dumas, R.,Hagen, G.,Guilfoyle, T.J.,Jez, J.M.,Strader, L.C.
Molecular basis for AUXIN RESPONSE FACTOR protein interaction and the control of auxin response repression.
Proc.Natl.Acad.Sci.USA, 111:5427-5432, 2014

PubMed Abstract: In plants, the AUXIN RESPONSE FACTOR (ARF) transcription factor family regulates gene expression in response to auxin. In the absence of auxin, ARF transcription factors are repressed by interaction with AUXIN/INDOLE 3-ACETIC ACID (Aux/IAA) proteins. Although the C termini of ARF and Aux/IAA proteins facilitate their homo- and heterooligomerization, the molecular basis for this interaction remained undefined. The crystal structure of the C-terminal interaction domain of Arabidopsis ARF7 reveals a Phox and Bem1p (PB1) domain that provides both positive and negative electrostatic interfaces for directional protein interaction. Mutation of interface residues in the ARF7 PB1 domain yields monomeric protein and abolishes interaction with both itself and IAA17. Expression of a stabilized Aux/IAA protein (i.e., IAA16) bearing PB1 mutations in Arabidopsis suggests a multimerization requirement for ARF protein repression, leading to a refined auxin-signaling model.

PubMed: 24706860
DOI: 10.1073/pnas.1400074111

Experimental method

X-RAY DIFFRACTION (2.4 Å)