4NJ6
PB1 Domain of AtARF7
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 19-ID |
Synchrotron site | APS |
Beamline | 19-ID |
Temperature [K] | 77 |
Detector technology | CCD |
Collection date | 2013-08-01 |
Detector | ADSC QUANTUM 315r |
Spacegroup name | H 3 |
Unit cell lengths | 150.591, 150.591, 183.616 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 33.650 - 2.400 |
R-factor | 0.211 |
Rwork | 0.209 |
R-free | 0.27100 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.008 |
RMSD bond angle | 1.272 |
Data reduction software | HKL-3000 |
Data scaling software | HKL-3000 |
Phasing software | PHASER |
Refinement software | PHENIX ((phenix.refine: 1.7.3_928)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 33.700 | 2.460 |
High resolution limit [Å] | 2.400 | 2.400 |
Number of reflections | 59901 | |
Completeness [%] | 98.7 | 99.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 6.5 | 277 | 25% PEG-6000, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 277K |