4NCB

Structure of Thermus thermophilus Argonaute bound to guide DNA and 19-mer target DNA with Mg2+

4NCB の概要

• エントリーDOI: 10.2210/pdb4ncb/pdb
• 関連するPDBエントリー: 4N41 4N47 4N76 4NCA
• 分子名称: Argonaute, 5'-D(P*TP*GP*AP*GP*GP*TP*AP*GP*TP*AP*GP*GP*TP*TP*GP*TP*AP*TP*AP*GP*T)-3', 5'-D(*TP*AP*TP*AP*CP*AP*AP*CP*C)-3', ... (8 entities in total)
• 機能のキーワード: argonaute, rna interference, dna interference, piwi, nuclear protein-dna complex, nuclear protein/dna
• 由来する生物種: Thermus thermophilus
• タンパク質・核酸の鎖数: 8
• 化学式量合計: 181480.54

構造登録者

Sheng, G.,Zhao, H.,Wang, J.,Rao, Y.,Wang, Y. (登録日: 2013-10-24, 公開日: 2014-01-15, 最終更新日: 2023-09-20)

主引用文献

Sheng, G.,Zhao, H.,Wang, J.,Rao, Y.,Tian, W.,Swarts, D.C.,van der Oost, J.,Patel, D.J.,Wang, Y.
Structure-based cleavage mechanism of Thermus thermophilus Argonaute DNA guide strand-mediated DNA target cleavage.
Proc.Natl.Acad.Sci.USA, 111:652-657, 2014

PubMed Abstract: We report on crystal structures of ternary Thermus thermophilus Argonaute (TtAgo) complexes with 5'-phosphorylated guide DNA and a series of DNA targets. These ternary complex structures of cleavage-incompatible, cleavage-compatible, and postcleavage states solved at improved resolution up to 2.2 Å have provided molecular insights into the orchestrated positioning of catalytic residues, a pair of Mg(2+) cations, and the putative water nucleophile positioned for in-line attack on the cleavable phosphate for TtAgo-mediated target cleavage by a RNase H-type mechanism. In addition, these ternary complex structures have provided insights into protein and DNA conformational changes that facilitate transition between cleavage-incompatible and cleavage-compatible states, including the role of a Glu finger in generating a cleavage-competent catalytic Asp-Glu-Asp-Asp tetrad. Following cleavage, the seed segment forms a stable duplex with the complementary segment of the target strand.

PubMed: 24374628
DOI: 10.1073/pnas.1321032111

実験手法

X-RAY DIFFRACTION (2.189 Å)